Versatile Bioconjugated Affibody-glutathione Transferase Protein Gate Keeper for Drug Delivery Nanoparticle

Abstract

Among the drug delivery carriers, mesoporous silica nanoparticles (MSN) have received wide attention due to their tunable pore morphology, high specific surface area, easy surface modification/ bioconjugation for targeting and physiochemical stability. They can host the therapeutic compounds due their void cores, as extra reservoir for drug storage. Also gatekeeper system can maximize the characterization of MSN for being used drug delivery systems. Although there are many kinds of gatekeeper systems ,like polymer, peptide, metal nanoparticles, not much improve in protein gate keeper. Protein gate keeper could be one of the most powerful platform by using direct interaction between receptor protein and affibody. Also our GST-affibody protein forms protein layer which can shield inside silica nanoparticle from unwished human serum protein (protein corona) Drug can be loaded into the pores of the carrier at large quantity. Recently, non-covalent protein gatekeepers have received significant attention towards encapsulation stability. Hence, we installed a biocompetable protein gatekeepers on the surface of drug loaded carriers. The GST-affibody possess important role in undergoing degradation in low pH. Further, this affibody can be conjugated with different types of antibody to target specific cancer cells to achieve a stable and biocompatible carrier for biomedical applications.