Co-utilization of glucose, xylose and cellobiosein E. coli and cellobiose biosensor applicable to farm feedstock

Abstract

Farms around the world produce large amounts of biomass. One of biomass is empty fruit bunches(EFB) and the most components of EFB are fibrous. EFB contains little chemical or external impurities. Therefore, if the pretreatment can be done properly, it can be processed biologically sufficiently. However, during consolidation bioprocessing(CBP) to decompose it, glucose (97.8g/L), xylose (13.0g/L), and other sugars are produced. And a small amount of cellobiose (13.0g/L) remains. Removing each of these requires a huge amount of time and cost. In addition, more pretreatment time is required to remove that low levels of cellobiose. Therefore, Our laboratory has developed E. coli that can consume all of them at the same time. And also we made the cellobiose plasmid biosensor which applicable to farm feedstock.