Recombinant protein-MOF hybrid complex

Abstract

Among the drug delivery carriers, MOF nanoparticle (metal organic framework) have received wide attention due to their tunable pore morphology, high specific surface area, easy surface modification and biodegradability in bio-environment. They can host the therapeutic compounds due their void cores, as extra reservoir for drug storage. Also gatekeeper system can maximize the characterization of MSN for being used drug delivery systems. Although there are many kinds of gatekeeper systems,like polymer, peptide, metal nanoparticles, not much improve in protein gate keeper. Protein gate keeper could be one of the most powerful platform by using direct interaction between receptor protein and affibody. Drug can be loaded into the pores of the carrier at large quantity. Recently, non-covalent protein gatekeepers have received significant attention towards encapsulation stability. Hence, we installed a biocompetable protein gatekeepers on the surface of drug loaded carriers. The GST-affibody possess important role in undergoing degradation in tumor environment. Further, this affibody can be conjugated with different types of antibody to target specific cancer cells to achieve a stable and biocompatible carrier for biomedical applications.Keywords: Glutathione; GST-affibody; Antibody; peptide gatekeepers; MOF nanoparticle , PCN-224; protein corona