Wide-Field Quantitative Phase Imaging for Tissue and Its Disease Model

Abstract

We present a label-free wide field phase imaging technique, which can replace the historical imaging. We have acquired phase-delay tissue of different type of major organ from mice. Not only healthy tissue, but also we able to have image of disease model such as acute kidney injury (AKI). AKI study has mainly conducted by histology, immunihistochemistry and western blot, wich only provide qualitative analysis. For better understanding of kidney structure in AKI, we present a new approach using digital holography conbined with wide-field scanning platform. Through the phase-delay information from the disease model, we were able to predict a stage of AKI based on distinct optical property such as scattering parameter and anisotropy. These retrieved optical parameters quantitatively demonstrate the healing process of AKI model of kidney with high contrast as well as its detailed anatomical structure.