Size-Selective Isolation of Urinary Exosome on Lab-on-a-Disc

Abstract

A size selective isolation of exosome is demonstrated on a centrifugal microfluidic system. Starting with 1 mL of raw urine sample, we suggest a rapid and easy method for isolation of exosomes by using nanosized filters integrated on a lab-on-a-disc. Our method is a cost effective, quick, and painless method for identification of the bacterium by analyzing the RNA in the exosomes secreted in the patient's urine. It may be used routinely for early diagnostics of hospitalized patients susceptible to sepsis, dramatically lowering its mortality rate.