14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, pp.1598 - 1600
Abstract
We present a diffusion-driven and long-range concentration gradient generator by using hydrogel as a porous membrane to prevent convection flows but allow the diffusion in a microfluidic device for the study of bacterial chemotaxis. Using the device, we characterize critical concentrations associated with chmotactic responses of cells that initially create a population band and then migrate in bands. In addition, we can find out the preferential chemotaxis of bacterial cells toward different carbon sources; glucose, galactose, mannose, over arabinose and xylose in this order, because the device can produce concentration gradients of multi-chemicals simultaneously as well.
Publisher
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010