Label-Free Electrochemical Detection of the p53 Core Domain Protein on Its Antibody Immobilized Electrode
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- Label-Free Electrochemical Detection of the p53 Core Domain Protein on Its Antibody Immobilized Electrode
- Yeo, Jongchan; Park, Jin-Young; Bae, Won Jin; Lee, Yoon Suk; Kim, Byeang Hyean; Cho, Yunje; Park, Su-Moon
- 18-crown-6; Antibody immobilized electrodes; Antibody molecules; Cancer markers; Concentration ranges; ELectrochemical detection; Electrochemical impedance; Gold disk electrodes; Human embryonic kidney cells; Label free; Modified electrodes; P53 protein; Quantitative result; Redox probe; Tumor suppressor proteins; Western blots
- Issue Date
- AMER CHEMICAL SOC
- ANALYTICAL CHEMISTRY, v.81, no.12, pp.4770 - 4777
- We report quantitative results on interactions between a tumor suppressor protein, p53, also known as a prognostic cancer marker, and its antibody. The p53 antibody molecules immobilized on an (R)-lipo-diaza-18-crown-6 self-assembled monolayer (SAM)-modified gold disk electrode were shown to effectively capture the p53 protein by Western blot, quartz crystal microbalance, and electrochemical impedance experiments. The p53 protein thus captured modulated the ability of the electrode for charge transfer to and from a redox probe in the solution in a p53 concentration range of similar to 0.1-30 mu g/mL. The same interaction was also observed in the human embryonic kidney cell lysate, demonstrating that the SAM-modified electrode can serve as a selective platform for electrochemically monitoring the cellular p53 concentration.
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