There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 434 | - |
dc.citation.startPage | 429 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 127 | - |
dc.contributor.author | Monnappa, Ajay Kalanjana | - |
dc.contributor.author | Lee, Siseon | - |
dc.contributor.author | Mitchell, Robert J. | - |
dc.date.accessioned | 2023-12-22T04:17:08Z | - |
dc.date.available | 2023-12-22T04:17:08Z | - |
dc.date.created | 2013-06-24 | - |
dc.date.issued | 2013-01 | - |
dc.description.abstract | A bioluminescent Escherichia coil bioreporter strain to detect hydrolysate related phenolics was developed by cloning the aaeXAB promoter from E. coli upstream of the luxCDABE genes. E. coli str. DH5 alpha carrying this plasmid (pDMA3) was responsive to sub-inhibitory concentrations of plant hydrolysate-related phenolics, such as ferulic and vanillic acids, responding to these compounds at concentrations as low as 9.8 and 4.9 mg/L, respectively. Experiments with a mixture of the compounds showed similar responses as with single compound tests, with a minimum detectable concentration of 19.5 mg/L Finally, tests using rice straw hydrolysates were conducted, with E. coli str. DH5 alpha/pDMA3 showing a maximum induction of 33-fold and a minimum detectable phenolic concentration of 9.3 mg/L, based upon Folin-Ciocalteu's reagent. These results demonstrate that this bioreporter maintains its sensitivity even with hydrolysate samples and that it can be potentially applied within biofuel industries to detect phenolics present within plant hydrolysates. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.127, pp.429 - 434 | - |
dc.identifier.doi | 10.1016/j.biortech.2012.09.086 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-84868334623 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/3397 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84868334623 | - |
dc.identifier.wosid | 000312926400058 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Sensing of plant hydrolysate-related phenolics with an aaeXAB::luxCDABE bioreporter strain of Escherichia coli | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.relation.journalResearchArea | Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Bioluminescence | - |
dc.subject.keywordAuthor | Lignin hydrolysate | - |
dc.subject.keywordAuthor | aaeA | - |
dc.subject.keywordAuthor | Ferulic acid | - |
dc.subject.keywordAuthor | Phenolic | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.