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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 118 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 109 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 24 | - |
dc.contributor.author | Nguyen, Nam Hoai | - |
dc.contributor.author | Kim, Jung-Rae | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T19:38:26Z | - |
dc.date.available | 2023-12-21T19:38:26Z | - |
dc.date.created | 2019-01-24 | - |
dc.date.issued | 2019-02 | - |
dc.description.abstract | In this study, whole-cell biosensors that detect and indicate the concentration of 3-hydroxypropionic acid (3-HP) by producing a green fluorescent signal were developed using a transcription factor (TF)-responsive 3- HP inducible promoter identified in the Pseudomonas denitrificans 3-HP degradation pathway. Upon forming a complex with 3-HP, the MmsR TF protein, a LysR-type transcriptional regulator (LTTR), binds to the PmmsA promoter and controls the expression of the PmmsA-regulated gfp reporter gene in response to the concentration of 3-HP. Furthermore, by enhancing the expression of MmsR and through mutagenesis of the PmmsA promoter region, three well-performing biosensors were developed that covered a wide dynamic range of 3-HP (0.01-100 mM when added externally) with ~100-fold signal change upon induction in P. denitrificans. The 3-HP biosensor machinery, composed of MmsR, the PmmsA promoter and gfp could also function well in E. coli and P. putida. The developed 3-HP biosensors should be useful for engineering 3-HP-producing strains and the enzymes associated with its production. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.24, no.1, pp.109 - 118 | - |
dc.identifier.doi | 10.1007/s12257-018-0380-8 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.scopusid | 2-s2.0-85059613092 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/33052 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs12257-018-0380-8 | - |
dc.identifier.wosid | 000461396500013 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Development of Biosensor for 3-Hydroxypropionic Acid | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | 3-hydroxypropionic acid | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | fluorescent protein | - |
dc.subject.keywordAuthor | Pseudomonas denitrificans | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
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