Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 164 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 157 | - |
dc.citation.title | SYNTHETIC AND SYSTEMS BIOTECHNOLOGY | - |
dc.citation.volume | 4 | - |
dc.contributor.author | Singh, Kalpana | - |
dc.contributor.author | Ainala, Satish Kumar | - |
dc.contributor.author | Kim, Yeonhee | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T18:40:55Z | - |
dc.date.available | 2023-12-21T18:40:55Z | - |
dc.date.created | 2020-02-20 | - |
dc.date.issued | 2019-09 | - |
dc.description.abstract | Lactic acid has two stereoisomers of D(−)- and L(+)-forms, both of which are important monomers of biodegradable plastic, poly-lactic acid. In this study, a novel D-lactate inducible system was identified in Pseudomonas fluorescens A506, partially characterized and tested as biosensor. The D-lactate catabolic operon (lldP-dld-II) was negatively regulated through the inversely transcribed D-lldR (encoding a GntR-type regulator), where the repression is relieved by addition of D-lactate. The derepression was specific to D-lactate and marginally affected by L-lactate. The D-LldR-responsive operator, showing dyad symmetry and separated by one base, was located between +11 and + 27 from the transcription start site of the lldP-dld-II operon. By site-directed mutagenesis, a motif with a dyad symmetry (AATTGGTAtTACCAATT), present in the upstream region of lldP, was identified as essential for the binding of LldR. D-lactate biosensors were developed by connecting the upregulation by D-lactate to a green fluorescent readout. About ~6.0-fold induction by 100 mM D-lactate was observed compared to L-lactate. | - |
dc.identifier.bibliographicCitation | SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, v.4, no.3, pp.157 - 164 | - |
dc.identifier.doi | 10.1016/j.synbio.2019.08.004 | - |
dc.identifier.issn | 2405-805X | - |
dc.identifier.scopusid | 2-s2.0-85071621730 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/33040 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S2405805X19300262?via%3Dihub | - |
dc.identifier.wosid | 000486446100008 | - |
dc.language | 영어 | - |
dc.publisher | KeAi Communications Co. | - |
dc.title | A novel D(−)-lactic acid-inducible promoter regulated by the GntR-family protein D-LldR of Pseudomonas fluorescens | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Enantiomer | - |
dc.subject.keywordAuthor | Lactic acid | - |
dc.subject.keywordAuthor | Repression | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.