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DC Field | Value | Language |
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dc.citation.startPage | 123332 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 309 | - |
dc.contributor.author | Cha, Daegeun | - |
dc.contributor.author | Ha, Hyo Seok | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-21T17:11:24Z | - |
dc.date.available | 2023-12-21T17:11:24Z | - |
dc.date.created | 2020-06-09 | - |
dc.date.issued | 2020-08 | - |
dc.description.abstract | Poly(3-hydroxybutyrate), a short-chain-length polyhydroxyalkanoate (scl-PHA), is considered as a good alternative to conventional synthetic plastics. However, various biopolymers with diverse characteristics are still in demand. In this study, four different types of scl-PHA were successfully produced by engineering levulinic acid (LA) utilization metabolic pathway and expressing heterologous PHA synthase (PhaEC), acetyl-CoA acetyltransferase (PhaA), and acetyl-CoA reductase (PhaB) in Pseudomonas putida EM42. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxyvalerate-co-4-hydroxyvalerate) [P(3HV-co-4HV)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxyvalerate) [P(3HB-co-3HV-co-4HV)] were produced by the natural LA pathway, poly(4-hydroxyvalerate) by lvaAB-deleted LA pathway, and P(3HV-co-4HV) and P(3HB-co-3HV-co-4HV) with relatively high 3HV by fadB-deleted LA pathway. PHA with different monomer fractions could be produced using different PHA synthases. Scl-PHA contents reached approximately 40% of cell dry mass under non-optimized flask culture. This demonstrates that the LA catabolic pathway may be a good alternative route to provide monomers for the production of various types of PHA. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.309, pp.123332 | - |
dc.identifier.doi | 10.1016/j.biortech.2020.123332 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-85083663018 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/32344 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0960852420306040 | - |
dc.identifier.wosid | 000568836400007 | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Metabolic engineering of Pseudomonas putida for the production of various types of short-chain-length polyhydroxyalkanoates from levulinic acid | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.relation.journalResearchArea | Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Biopolymer | - |
dc.subject.keywordAuthor | Levulinic acid catabolism | - |
dc.subject.keywordAuthor | PHA synthase | - |
dc.subject.keywordAuthor | Polyhydroxyalkanoates | - |
dc.subject.keywordAuthor | Pseudomonas putida | - |
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