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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 22 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 15 | - |
dc.citation.title | CYTOMETRY | - |
dc.citation.volume | 13 | - |
dc.contributor.author | AMBLARD, F | - |
dc.contributor.author | CANTIN, C | - |
dc.contributor.author | DURAND, J | - |
dc.contributor.author | FISCHER, A | - |
dc.contributor.author | SEKALY, R | - |
dc.contributor.author | AUFFRAY, C | - |
dc.date.accessioned | 2023-12-22T13:06:56Z | - |
dc.date.available | 2023-12-22T13:06:56Z | - |
dc.date.created | 2020-01-31 | - |
dc.date.issued | 1992-20 | - |
dc.description.abstract | When analyzed in a flow cytometer, particles are suddenly accelerated to high velocities (1-10 m.s-1) over very short distances. This feature is essential to obtain high analysis rates and low coincidence levels, but translates into very strong velocity gradients (> 10(5) s-1): particles experience strong hydrodynamic stresses that elongate them and tend to dissociate weakly associated complexes. In order to analyze fragile conjugates formed by heterotypic adhesion between two cell types, a flow cytometer was modified to make hydrodynamic stress not only much weaker but also adjustable. A new and easily adaptable flow cell was designed for the instruments of the FACS(TM) series; it provided satisfactory hydrodynamic conditions on a wide continous range of flow rates. Accompanying electronic adaptations permitted standard analysis between 0.01 and 10 m.s-1. At 0.01 m.s-1, the velocity gradient roughly amounts to 50 s-1. Conjugates formed by the adhesion between human B and resting T lymphocytes, disrupted. in conventional flow cytometers, could be detected and precisely quantified provided analysis velocity was kept below 0.1 m.s-1. We conclude that low velocity flow cytometry makes possible the quantification of weak intercellular adhesion phenomena, and is potentially useful for the future development of new biomechanical techniques and other applications. | - |
dc.identifier.bibliographicCitation | CYTOMETRY, v.13, no.1, pp.15 - 22 | - |
dc.identifier.doi | 10.1002/cyto.990130105 | - |
dc.identifier.issn | 0196-4763 | - |
dc.identifier.scopusid | 2-s2.0-0026543120 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/31108 | - |
dc.identifier.wosid | A1992GX31100003 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-LISS | - |
dc.title | NEW CHAMBER FOR FLOW CYTOMETRIC ANALYSIS OVER AN EXTENDED RANGE OF STREAM-VELOCITY AND APPLICATION TO CELL-ADHESION MEASUREMENTS | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Cell Biology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Cell Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | LOW VELOCITY FLOW CYTOMETRY | - |
dc.subject.keywordAuthor | FLOW CHAMBER | - |
dc.subject.keywordAuthor | FLUID MECHANICS | - |
dc.subject.keywordAuthor | HYDRODYNAMIC STRESS | - |
dc.subject.keywordAuthor | HYDRODYNAMIC FOCUSING | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | LYMPHOCYTE ADHESION | - |
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