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DC Field | Value | Language |
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dc.citation.endPage | 848 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 842 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 273 | - |
dc.contributor.author | Wang, JS | - |
dc.contributor.author | Dong, XW | - |
dc.contributor.author | Myung, KJ | - |
dc.contributor.author | Hendrickson, EA | - |
dc.contributor.author | Reeves, WH | - |
dc.date.accessioned | 2023-12-22T12:36:30Z | - |
dc.date.available | 2023-12-22T12:36:30Z | - |
dc.date.created | 2020-01-31 | - |
dc.date.issued | 1998-01 | - |
dc.description.abstract | The Ku autoantigen is a heterodimer of 70 (p70) and similar to 80 kDa (p80) subunits that is the DNA-binding component of the DNA-dependent protein kinase (DNA-PK) complex involved in DNA repair and V(D)J recombination. Binding to DNA ends is critical to the function of DNA-PK, but how Ku interacts with DNA is not completely understood. To define the role of p70 and p80 and their dimerization in DNA binding, heterodimers were assembled by co-expressing the subunits using recombinant baculoviruses, Two p70 dimerization sites, amino acids 1-115 and 430-482, respectively, mere identified, Binding of p70 to linear double-stranded DNA could be demonstrated by an immunoprecipitation assay, and required the C-terminal portion (amino acids 430-609), but not interaction with p80, The p70 mutants 1-600, 1-542, 1-115, and 430-600 did not bind DNA efficiently, However, DNA binding of 1-600, 1-542, and 1-115, but not 430-600, was restored by dimerization with p80, indicating that p70 has two DNA binding sites, each partially overlapping one of the dimerization sites, The C-terminal domain can bind DNA by itself, but the N-terminal domain requires dimerization with p80, These observations could be relevant to the multiple functional activities of Ku and explain controversies regarding the role of dimerization in DNA binding. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.273, no.2, pp.842 - 848 | - |
dc.identifier.doi | 10.1074/jbc.273.2.842 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-0031974492 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/31101 | - |
dc.identifier.url | https://www.jbc.org/content/273/2/842 | - |
dc.identifier.wosid | 000071411400029 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Identification of two domains of the p70 Ku protein mediating dimerization with p80 and DNA binding | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | V(D)J RECOMBINATION | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | SUBUNIT | - |
dc.subject.keywordPlus | PRODUCT | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | CDNA | - |
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