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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 10684 | - |
dc.citation.number | 38 | - |
dc.citation.startPage | 10678 | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 67 | - |
dc.contributor.author | Kim, Doyun | - |
dc.contributor.author | Sathesh-Prabu, Chandran | - |
dc.contributor.author | JooYeon, Young | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-21T18:41:26Z | - |
dc.date.available | 2023-12-21T18:41:26Z | - |
dc.date.created | 2019-10-22 | - |
dc.date.issued | 2019-09 | - |
dc.description.abstract | gamma-Hydroxyvalerate (4HV) is an important monomer used to produce various valuable polymers and products. In this study, an engineered 3-hydroxybutyrate dehydrogenase that can convert levulinic acid (LA) into 4HV was co-expressed with a cofactor (NADH) regeneration system mediated by an NAD(+)-dependent formate dehydrogenase (CbFDH) in the Escherichia coli strain, MG1655. The resulting strain produced 23-fold more 4HV in a shake flask. The 4HV production was not dependent on ATP and required low aeration; all of these are considered beneficial characteristics for the production of target compounds, especially at an industrial scale. Under optimized conditions in a 5 L fermenter, the titer, productivity, and molar conversion efficiency for 4HV reached 100 g/L, 4.2 g/L/h, and 92%, respectively. Our system could prove to be a promising method for the large-scale production of 4HV from LA at low-cost and using a renewable biomass source. | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.67, no.38, pp.10678 - 10684 | - |
dc.identifier.doi | 10.1021/acs.jafc.9b04304 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.scopusid | 2-s2.0-85072627472 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/30745 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acs.jafc.9b04304 | - |
dc.identifier.wosid | 000488322400014 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | High-Level Production of 4-Hydroxyvalerate from Levulinic Acid via Whole-Cell Biotransformation Decoupled from Cell Metabolism | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology | - |
dc.relation.journalResearchArea | Agriculture; Chemistry; Food Science & Technology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 4-hydroxyvalerate | - |
dc.subject.keywordAuthor | levulinic acid | - |
dc.subject.keywordAuthor | 3-hydroxybutyrate dehydrogenase (3HBDH) | - |
dc.subject.keywordAuthor | formate dehydrogenase (FDH) | - |
dc.subject.keywordPlus | COMPLETE GENOME SEQUENCE | - |
dc.subject.keywordPlus | FED-BATCH CULTIVATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GAMMA-VALEROLACTONE | - |
dc.subject.keywordPlus | EFFICIENT UTILIZATION | - |
dc.subject.keywordPlus | BIOCATALYSIS | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | CHALLENGES | - |
dc.subject.keywordPlus | REDUCTION | - |
dc.subject.keywordPlus | PLATFORM | - |
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