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이성국

Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Lab.
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High-Level Production of 4-Hydroxyvalerate from Levulinic Acid via Whole-Cell Biotransformation Decoupled from Cell Metabolism

Author(s)
Kim, DoyunSathesh-Prabu, ChandranJooYeon, YoungLee, Sung Kuk
Issued Date
2019-09
DOI
10.1021/acs.jafc.9b04304
URI
https://scholarworks.unist.ac.kr/handle/201301/30745
Fulltext
https://pubs.acs.org/doi/10.1021/acs.jafc.9b04304
Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.67, no.38, pp.10678 - 10684
Abstract
gamma-Hydroxyvalerate (4HV) is an important monomer used to produce various valuable polymers and products. In this study, an engineered 3-hydroxybutyrate dehydrogenase that can convert levulinic acid (LA) into 4HV was co-expressed with a cofactor (NADH) regeneration system mediated by an NAD(+)-dependent formate dehydrogenase (CbFDH) in the Escherichia coli strain, MG1655. The resulting strain produced 23-fold more 4HV in a shake flask. The 4HV production was not dependent on ATP and required low aeration; all of these are considered beneficial characteristics for the production of target compounds, especially at an industrial scale. Under optimized conditions in a 5 L fermenter, the titer, productivity, and molar conversion efficiency for 4HV reached 100 g/L, 4.2 g/L/h, and 92%, respectively. Our system could prove to be a promising method for the large-scale production of 4HV from LA at low-cost and using a renewable biomass source.
Publisher
AMER CHEMICAL SOC
ISSN
0021-8561
Keyword (Author)
4-hydroxyvaleratelevulinic acid3-hydroxybutyrate dehydrogenase (3HBDH)formate dehydrogenase (FDH)
Keyword
COMPLETE GENOME SEQUENCEFED-BATCH CULTIVATIONESCHERICHIA-COLIGAMMA-VALEROLACTONEEFFICIENT UTILIZATIONBIOCATALYSISSPECIFICITYCHALLENGESREDUCTIONPLATFORM

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