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Park, Jongnam
Materials and Chemistry Lab (MCL)
Research Interests
  • Inorganic synthesis, microfluidics, nanomaterials, surface engineering, bioimaging


Incorporation of Thrombin Cleavage Peptide into a Protein Cage for Constructing a Protease-Responsive Multifunctional Delivery Nanoplatform

DC Field Value Language Kang, Young Ji ko Park, Dae Cheul ko Shin, Hyun-Hee ko Park, Jongnam ko Kang, Sebyung ko 2014-04-10T01:27:25Z - 2013-06-12 ko 2012-12 ko
dc.identifier.citation BIOMACROMOLECULES, v.13, no.12, pp.4057 - 4064 ko
dc.identifier.issn 1525-7797 ko
dc.identifier.uri -
dc.description.abstract Protein cages are spherical hollow supramolecules that are attractive nanoscale platforms for constructing cargo delivery vehicles. Using ferritin isolated from the hyperthermophilic archaeon Pyrococcus furiosus (Pf_Fn), we developed a multifunctional protein cage-based delivery nanoplatform that can hold cargo molecules securely, deliver them to the targeted sites, and release them to the targeted cells. The release is triggered by cleavage induced by the protease, thrombin. The thrombin cleavage peptide (GGLVPR/GSGAS) was inserted into the flexible loop region of Pf_Fn, which is located at a 4-fold axis of symmetry exposed on the surface of protein cages (Thr-Pf_Fn). Subsequently, the C-terminal glycine, which is situated in the interior cavity, was substituted with cysteine (G173C) to permit site-specific conjugation of cargo molecules. The introduced cysteine (G173C) was labeled with a fluorescent probe (F5M-Thr-Pf_Fn) for cell imaging and cargo release monitoring. The surface of F5M-Thr-Pf_Fn was further modified with biotins (F5M-Thr-Pf_Fn-NPB) as targeting ligands. The specific binding of dual functionalized F5M-Thr-Pf_Fn-NPB to the MDA MB 231 cell line, which overexpresses biotin-specific receptors on its surface, was confirmed by fluorescence microscopic analyses. The inserted thrombin cleavage peptides were effectively cleaved by thrombin, resulting in the release of the C-terminal helix in buffer and on the targeted cells without disruption of the cage architecture. Protein cage scaffolds that combine genetic and chemical modifications may serve as stimulus-responsive delivery nanoplatforms and provide opportunities for developing new types of theranostic nanoplatforms. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher AMER CHEMICAL SOC ko
dc.title Incorporation of Thrombin Cleavage Peptide into a Protein Cage for Constructing a Protease-Responsive Multifunctional Delivery Nanoplatform ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84870931671 ko
dc.identifier.wosid 000312035000019 ko
dc.type.rims ART ko
dc.description.wostc 7 *
dc.description.scopustc 7 * 2015-02-28 * 2014-08-26 *
dc.identifier.doi 10.1021/bm301339s ko
dc.identifier.url ko
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