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- Kwon, Taejoon
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Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate
- Author(s)
- Kim, Beomsue, Fukuda, Masahiro, Lee, Jung-Yeol, Su, Dongdong, Sanu, Srikanta, Silvin, Aymeric, Khoo, Audrey T. T., Kwon, Taejoon, Liu, Xiao, Chi, Weijie, Liu, Xiaogang, Choi, Sejong, Wan, Diana S. Y., Park, Sung-Jin, Kim, Jin-Soo, Ginhoux, Florent, Je, H. Shawn, Chang, Young-Tae
- Issued Date
- 2019-06
- Citation
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.58, no.24, pp.7972 - 7976
- Abstract
- Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
- Publisher
- WILEY-V C H VERLAG GMBH
- ISSN
- 1433-7851
- Keyword (Author)
- biological activity, fluorescent probes, imaging agents, microglia, structure-activity relationships
- Keyword
- CELLS, TRANSCRIPTOME
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