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MitchellRobertJames

Mitchell, Robert J.
Applied and Environmental Microbiology Lab.
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dc.citation.endPage 2247 -
dc.citation.number 6 -
dc.citation.startPage 2242 -
dc.citation.title JOURNAL OF THE AMERICAN CHEMICAL SOCIETY -
dc.citation.volume 135 -
dc.contributor.author Byun, Chang Kyu -
dc.contributor.author Hwang, Hyundoo -
dc.contributor.author Choi, Woon Sun -
dc.contributor.author Yaguchi, Toshiyuki -
dc.contributor.author Park, Jiwoon -
dc.contributor.author Kim, Dasol -
dc.contributor.author Mitchell, Robert J. -
dc.contributor.author Kim, Taesung -
dc.contributor.author Cho, Yoon-Kyoung -
dc.contributor.author Takayama, Shuichi -
dc.date.accessioned 2023-12-22T04:13:37Z -
dc.date.available 2023-12-22T04:13:37Z -
dc.date.created 2013-06-10 -
dc.date.issued 2013-02 -
dc.description.abstract This paper describes a system to study how small physical perturbations can affect bacterial community behavior in unexpected ways through modulation of diffusion and convective transport of chemical communication molecules and resources. A culture environment that mimics the chemically open characteristic of natural bacterial habitats but with user-defined spatiotemporal control of bacteria microcolonies is realized through use of an aqueous two phase system (ATPS). The ATPS is formulated with nontoxic dextran (DEX) and poly(ethylene glycol) (PEG) dissolved in cell culture media. DEX-phase droplets formed within a bulk PEG-phase stably confine the bacteria within it while small molecules diffuse relatively freely. Bacteria-containing DEX droplets can also be magnetically relocated, without loss of its bacterial content, when DEX-conjugated magnetic particles are included. We found that decreasing the distance between quorum-sensing (QS)-coupled microcolonies increased green fluorescent protein (GFP) expression due to increased inter-colony chemical communication but with upper limits. Periodic relocation of the chemical signal receiver colony, however, increased GFP expression beyond these typical bounds predicted by quorum sensing concepts alone by maintaining inter-colony chemical communication while also relieving the colony of short-range resource depletion effects. Computer simulations suggest that such increased productive output in response to periodic nonlethal physical perturbations is a common feature of chemically activated interactive cell systems where there is also a short-range inhibitory effect. In addition to providing insights on the effect of bacteria relocation, the magnetic ATPS droplet manipulation capability should be broadly useful for bioanalyses applications where selective partitioning at the microscale in fully aqueous conditions is needed -
dc.identifier.bibliographicCitation JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.135, no.6, pp.2242 - 2247 -
dc.identifier.doi 10.1021/ja3094923 -
dc.identifier.issn 0002-7863 -
dc.identifier.scopusid 2-s2.0-84873632257 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/2961 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84873632257 -
dc.identifier.wosid 000315080100043 -
dc.language 영어 -
dc.publisher AMER CHEMICAL SOC -
dc.title Productive Chemical Interaction between a Bacterial Microcolony Couple Is Enhanced by Periodic Relocation -
dc.type Article -
dc.relation.journalWebOfScienceCategory Chemistry, Multidisciplinary -
dc.relation.journalResearchArea Chemistry -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordPlus PROTEIN -
dc.subject.keywordPlus DIFFUSION -
dc.subject.keywordPlus PATTERNS -
dc.subject.keywordPlus COMMUNICATION -
dc.subject.keywordPlus ESCHERICHIA-COLI -
dc.subject.keywordPlus VIBRIO-FISCHERI -
dc.subject.keywordPlus CELL-DENSITY -
dc.subject.keywordPlus LOW PH -
dc.subject.keywordPlus QUORUM -
dc.subject.keywordPlus EXPRESSION -

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