File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Full metadata record

DC Field Value Language
dc.citation.endPage 7576 -
dc.citation.number 9 -
dc.citation.startPage 7567 -
dc.citation.title JOURNAL OF BIOLOGICAL CHEMISTRY -
dc.citation.volume 286 -
dc.contributor.author Kim, Ji Hae -
dc.contributor.author Lee, Jung Ok -
dc.contributor.author Lee, Soo Kyung -
dc.contributor.author Moon, Ji Wook -
dc.contributor.author You, Ga Young -
dc.contributor.author Kim, Su Jin -
dc.contributor.author Park, Sun-Hwa -
dc.contributor.author Park, Ji Man -
dc.contributor.author Lim, Se Young -
dc.contributor.author Suh, Pann-Ghill -
dc.contributor.author Uhm, Kyung-Ok -
dc.contributor.author Song, Min Seok -
dc.contributor.author Kim, Hyeon Soo -
dc.date.accessioned 2023-12-22T06:36:10Z -
dc.date.available 2023-12-22T06:36:10Z -
dc.date.created 2013-06-05 -
dc.date.issued 2011-03 -
dc.description.abstract Homocysteine sulfinic acid (HCSA) is a homologue of the amino acid cysteine and a selective metabotropic glutamate receptor (mGluR) agonist. However, the metabolic role of HCSA is poorly understood. In this study, we showed that HCSA and glutamate stimulated glucose uptake in C2C12 mouse myoblast cells and increased AMP-activated protein kinase (AMPK) phosphorylation. RT-PCR and Western blot analysis revealed that C2C12 expresses mGluR5. HCSA transiently increased the intracellular calcium concentration. Although alpha-methyl-4-carboxyphenylglycine, a metabotropic glutamate receptor antagonist, blocked the action of HCSA in intracellular calcium response and AMPK phosphorylation, 6-cyano-7-nitroquinoxaline-2,3-dione, an AMPA antagonist, did not exhibit such effects. Knockdown of mGluR5 with siRNA blocked HCSA-induced AMPK phosphorylation. Pretreatment of cells with STO-609, a calmodulin-dependent protein kinase kinase (CaMKK) inhibitor, blocked HCSA-induced AMPK phosphorylation, and knockdown of CaMKK blocked HCSA-induced AMPK phosphorylation. In addition, HCSA activated p38 mitogen-activated protein kinase (MAPK). Expression of dominant-negative AMPK suppressed HCSA-mediated phosphorylation of p38 MAPK, and inhibition of AMPK and p38 MAPK blocked HCSA-induced glucose uptake. Phosphorylation of protein kinase C zeta (PKC zeta) was also increased by HCSA. Pharmacologic inhibition or knockdown of p38 MAPK blocked HCSA-induced PKC zeta phosphorylation, and knockdown of PKC zeta suppressed the HCSA-induced increase of cell surface GLUT4. The stimulatory effect of HCSA on cell surface GLUT4 was impaired in FITC-conjugated PKC zeta siRNA-transfected cells. Together, the above results suggest that HCSA may have a beneficial role in glucose metabolism in skeletal muscle cells via stimulation of AMPK. -
dc.identifier.bibliographicCitation JOURNAL OF BIOLOGICAL CHEMISTRY, v.286, no.9, pp.7567 - 7576 -
dc.identifier.doi 10.1074/jbc.M110.149328 -
dc.identifier.issn 0021-9258 -
dc.identifier.scopusid 2-s2.0-79953190054 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/2923 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79953190054 -
dc.identifier.wosid 000287737300070 -
dc.language 영어 -
dc.publisher AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC -
dc.title The Glutamate Agonist Homocysteine Sulfinic Acid Stimulates Glucose Uptake through the Calcium-dependent AMPK-p38 MAPK-Protein Kinase C zeta Pathway in Skeletal Muscle Cells -
dc.type Article -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.