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DC Field | Value | Language |
---|---|---|
dc.citation.number | 5 | - |
dc.citation.startPage | 051204 | - |
dc.citation.title | JOURNAL OF BIOMEDICAL OPTICS | - |
dc.citation.volume | 19 | - |
dc.contributor.author | Chang, Minhyeok | - |
dc.contributor.author | Kwon, Mijin | - |
dc.contributor.author | Kim, Sooran | - |
dc.contributor.author | Yunn, Na-Oh | - |
dc.contributor.author | Kim, Daehyung | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.contributor.author | Lee, Jong-Bong | - |
dc.date.accessioned | 2023-12-22T02:42:04Z | - |
dc.date.available | 2023-12-22T02:42:04Z | - |
dc.date.created | 2014-01-20 | - |
dc.date.issued | 2014-05 | - |
dc.description.abstract | We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-β-cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOMEDICAL OPTICS, v.19, no.5, pp.051204 | - |
dc.identifier.doi | 10.1117/1.JBO.19.5.051204 | - |
dc.identifier.issn | 1083-3668 | - |
dc.identifier.scopusid | 2-s2.0-84891528219 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/2842 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84891528219 | - |
dc.identifier.wosid | 000338334600005 | - |
dc.language | 영어 | - |
dc.publisher | SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS | - |
dc.title | Aptamer-based single-molecule imaging of insulin receptors in living cells | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Optics; Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Optics; Radiology, Nuclear Medicine & Medical Imaging | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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