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Aptamer-based single-molecule imaging of insulin receptors in living cells

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Title
Aptamer-based single-molecule imaging of insulin receptors in living cells
Author
Chang, MinhyeokKwon, MijinKim, SooranYunn, Na-OhKim, DaehyungRyu, Sung HoLee, Jong-Bong
Keywords
aptamer; cholesterol; diffusion; insulin receptor; single-molecule imaging; total internal reflection fluorescence microscopy
Issue Date
2014-05
Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Citation
JOURNAL OF BIOMEDICAL OPTICS, v.19, no.5, pp.051204 -
Abstract
We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-β-cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane.
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DOI
10.1117/1.JBO.19.5.051204
ISSN
1083-3668
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SLS_Journal Papers
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