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DC Field | Value | Language |
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dc.citation.endPage | 67 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 61 | - |
dc.citation.title | TISSUE ENGINEERING PART C-METHODS | - |
dc.citation.volume | 17 | - |
dc.contributor.author | Torisawa, Yu-Suke | - |
dc.contributor.author | Mosadegh, Bobak | - |
dc.contributor.author | Cavnar, Stephen P. | - |
dc.contributor.author | Ho, Mitchell | - |
dc.contributor.author | Takayama, Shuichi | - |
dc.date.accessioned | 2023-12-22T06:37:25Z | - |
dc.date.available | 2023-12-22T06:37:25Z | - |
dc.date.created | 2013-06-24 | - |
dc.date.issued | 2011-01 | - |
dc.description.abstract | This article describes a simple and rapid cell patterning method to form co-culture microarrays in commercially available Transwells. A thin poly(dimethylsiloxane) (PDMS) layer is printed on the underside of a Transwell using a PDMS stamp. Arbitrary cellular patterns are generated according to the geometric features of the thin PDMS layer through hydrodynamic forces that guide cells onto the membrane only over the PDMS-uncoated regions. Micropatterns of surface-adhered cells (we refer to this as two-dimensional) or non-surface-adhered clusters of cells (we refer to this as three-dimensional) can be generated depending on the surface treatment of the filter membrane. Additionally, co-cultures can be established by introducing different types of cells on the membrane or in the bottom chamber of the Transwell. We show that this co-culture method can evaluate mouse embryonic stem (mES) cell differentiation based on heterogeneous cell-cell interactions. Co-culture of mES cells and HepG2 cells decreased SOX17 expression of mES cells, and direct cell-cell contact further decreased SOX17 expression, indicating that co-culture with HepG2 cells inhibits endoderm differentiation through soluble factors and cell-cell contact. This method is simple and user-friendly and should be broadly useful to study cell shapes and cell-cell interactions | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART C-METHODS, v.17, no.1, pp.61 - 67 | - |
dc.identifier.doi | 10.1089/ten.tec.2010.0305 | - |
dc.identifier.issn | 1937-3384 | - |
dc.identifier.scopusid | 2-s2.0-78650901976 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/2834 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78650901976 | - |
dc.identifier.wosid | 000285874700007 | - |
dc.language | 영어 | - |
dc.publisher | MARY ANN LIEBERT INC | - |
dc.title | Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering; Cell Biology; Engineering, Biomedical; Materials Science, Biomaterials | - |
dc.relation.journalResearchArea | Cell Biology; Engineering; Materials Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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