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Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1544 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1535 | - |
dc.citation.title | PROTEIN SCIENCE | - |
dc.citation.volume | 25 | - |
dc.contributor.author | Min, Duyoung | - |
dc.contributor.author | Arbing, Mark A. | - |
dc.contributor.author | Jefferson, Robert E. | - |
dc.contributor.author | Bowie, James U. | - |
dc.date.accessioned | 2023-12-21T23:16:00Z | - |
dc.date.available | 2023-12-21T23:16:00Z | - |
dc.date.created | 2019-10-04 | - |
dc.date.issued | 2016-08 | - |
dc.description.abstract | Manipulating single molecules and systems of molecules with mechanical force is a powerful technique to examine their physical properties. Applying force requires attachment of the target molecule to larger objects using some sort of molecular tether, such as a strand of DNA. DNA handle attachment often requires difficult manipulations of the target molecule, which can preclude attachment to unstable, hard to obtain, and/or large, complex targets. Here we describe a method for covalent DNA handle attachment to proteins that simply requires the addition of a preprepared reagent to the protein and a short incubation. The handle attachment method developed here provides a facile approach for studying the biomechanics of biological systems. | - |
dc.identifier.bibliographicCitation | PROTEIN SCIENCE, v.25, no.8, pp.1535 - 1544 | - |
dc.identifier.doi | 10.1002/pro.2952 | - |
dc.identifier.issn | 0961-8368 | - |
dc.identifier.scopusid | 2-s2.0-84979055380 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/27805 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/full/10.1002/pro.2952 | - |
dc.identifier.wosid | 000380068700017 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | A simple DNA handle attachment method for single molecule mechanical manipulation experiments | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | forced unfolding | - |
dc.subject.keywordAuthor | magnetic tweezers | - |
dc.subject.keywordAuthor | optical tweezers | - |
dc.subject.keywordAuthor | SpyTag | - |
dc.subject.keywordAuthor | SpyCatcher | - |
dc.subject.keywordAuthor | protein folding | - |
dc.subject.keywordAuthor | membrane protein | - |
dc.subject.keywordPlus | FORCE SPECTROSCOPY | - |
dc.subject.keywordPlus | PEPTIDE TAG | - |
dc.subject.keywordPlus | ENERGY LANDSCAPES | - |
dc.subject.keywordPlus | SNARE COMPLEX | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | MACHINE | - |
dc.subject.keywordPlus | TENSION | - |
dc.subject.keywordPlus | BOND | - |
dc.subject.keywordPlus | NSF | - |
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