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LAB-ON-A-DISCS FOR QUANTIFICATION OF MICROALGAL LIPIDS AND NATURAL ANTIOXIDANTS OF BEVERAGE SAMPLES

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Title
LAB-ON-A-DISCS FOR QUANTIFICATION OF MICROALGAL LIPIDS AND NATURAL ANTIOXIDANTS OF BEVERAGE SAMPLES
Author
Kim, Yubin
Advisor
Cho, Yoon-Kyoung
Issue Date
2019-08
Publisher
Graduate School of UNIST
Abstract
Since the first introduction in 1960s, lab-on-a-disc platform has gained much attention due to its great advantages such as simple operation, rapid reaction, low cost, full integration and automation. Lab-on-a-disc has been applied for various research fields such as biomedical, environmental, energy and food agricultural field. In this thesis, fully integrated lab-on-a-disc platform was demonstrated for quantification of microalgal lipid and natural antioxidants from beverage samples, and disc analyzer was introduced to operate the on-disc optical detection. For microalgae lipid quantification, fully automated lab-on-a-disc platform was developed for rapid on-site quantification of lipids from microalgal samples. The whole serial process for microalgal lipid quantification was integrated on lab-on-a-disc platform. Lab-on-a-disc operation time was 13 min. To integrate liquid-solvent extraction of microalgal lipid on a disc, the organic solvent compatible (for ethanol and n-hexane) lab-on-a-disc was newly fabricated. Fabrication technique was developed by combining thermal fusion bonding and laser printed carbon dot based valving. For natural antioxidant determination from beverage samples, lab-on-a-disc platform was developed to integrate the complicated determination steps of antioxidant activity (AA) and the total phenolic content (TPC) from beverage sample. Different beverage samples including wine, beer, various fruit juices and tea were analyzed using our lab-on-a-disc platform. For disc analyzer, on-disc optical signal detecting instrument was developed. It can detect on-disc signal of absorbance and chemiluminescence from result solution. The trials and errors to develop the disc analyzer for following researchers was demonstrated. From both lab-on-a-disc quantification methods, it is proved that lab-on-a-disc can be effectively used for quantification of microalgal biofuel and food antioxidants. It is expected that our lab-on-a-disc based microalgal lipid quantification works give considerable contribution to the commercial production of microalgal-based biofuels by providing rapid, cost-effective, user-friendly on-site quantification of microalgal lipids. Also, our on-disc natural antioxidant detection works would also contribute to functional beverage industry as providing fast, simple determination of natural antioxidants from beverage sample. Both lab-on-a-disc was fabricated by the organic solvent compatible (for ethanol and n-hexane) manner, and it can be used for chemical engineering fields for quality control.
Description
Department of Chemical Engineering
URI
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ECHE_Theses_Ph.D.
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