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Kim, Taesung
Microfluidics & Nanomechatronics Lab (μFNM)
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  • Micro-/Nanofluidics

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Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor

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Title
Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor
Author
Lee, Jae MyungLee, JunhyeongKim, TaesungLee, Sung Kuk
Keywords
GREEN FLUORESCENT PROTEIN; HETEROLOGOUS PROTEINS; PHAGE-LAMBDA; BACTERIA; LIGHT; PROMOTER; SYSTEM; ACTIVATION; CELLS; K-12
Issue Date
2013-01
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v.8, no.1, pp.1 - 8
Abstract
We present a light-switchable gene expression system for both inducible and switchable control of gene expression at a single cell level in Escherichia coli using a previously constructed light-sensing system. The lambda cl repressor gene with an LVA degradation tag was expressed under the control of the ompC promoter on the chromosome. The green fluorescent protein (GFP) gene fused to a lambda repressor-repressible promoter was used as a reporter. This light-switchable system allows rapid and reversible induction or repression of expression of the target gene at any desired time. This system also ensures homogenous expression across the entire cell population. We also report the design of a miniaturized photobioreactor to be used in combination with the light-switchable gene expression system. The miniaturized photobioreactor helps to reduce unintended induction of the light receptor due to environmental disturbances and allows precise control over the duration of induction. This system would be a good tool for switchable, homogenous, strong, and highly regulatable expression of target genes over a wide range of induction times. Hence, it could be applied to study gene function, optimize metabolic pathways, and control biological systems both spatially and temporally.
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DOI
10.1371/journal.pone.0052382
ISSN
1932-6203
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MNE_Journal Papers
ECHE_Journal Papers
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