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Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics
  • Bio-Nanotechnology
  • Biofluids
  • Biomaterials
  • Tissue Engineering and Regenerative Medicine.

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Patterning Bacterial Communities on Epithelial Cells

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dc.contributor.author Dwidar, Mohammed ko
dc.contributor.author Leung, Brendan M. ko
dc.contributor.author Yaguchi, Toshiyuki ko
dc.contributor.author Takayama, Shuichi ko
dc.contributor.author Mitchell, Robert J. ko
dc.date.available 2014-04-09T08:35:06Z -
dc.date.created 2013-08-28 ko
dc.date.issued 2013-06 ko
dc.identifier.citation PLOS ONE, v.8, no.6, pp.1 - 14 ko
dc.identifier.issn 1932-6203 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/2696 -
dc.description.abstract Micropatterning of bacteria using aqueous two phase system (ATPS) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. This method was used to compare the effects of Escherichia coli strain MG1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from Yersinia pseudotuberculosis on the underlying epithelial cell layer. Large portions of the cell layer beneath the invasive strain were killed or detached while the non-invasive E. coli had no apparent effect on the epithelial cell layer over a 24 h observation period. In addition, simultaneous testing of the localized effects of three different bacterial species; E. coli MG1655, Shigella boydii KACC 10792 and Pseudomonas sp DSM 50906 on an epithelial cell layer is also demonstrated. The paper further shows the ability to use a bacterial predator, Bdellovibrio bacteriovorus HD 100, to selectively remove the E. coli, S. boydii and P. sp communities from this bacteria-patterned epithelial cell layer. Importantly, predation and removal of the P. Sp was critical for maintaining viability of the underlying epithelial cells. Although this paper focuses on a few specific cell types, the technique should be broadly applicable to understand a variety of bacteria-epithelial cell interactions ko
dc.description.statementofresponsibility open -
dc.language 영어 ko
dc.publisher PUBLIC LIBRARY SCIENCE ko
dc.title Patterning Bacterial Communities on Epithelial Cells ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84879048810 ko
dc.identifier.wosid 000321038800005 ko
dc.type.rims ART ko
dc.description.wostc 3 *
dc.description.scopustc 2 *
dc.date.tcdate 2015-02-28 *
dc.date.scptcdate 2014-07-12 *
dc.identifier.doi 10.1371/journal.pone.0067165 ko
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84879048810 ko
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