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DC Field | Value | Language |
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dc.citation.endPage | 376 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 361 | - |
dc.citation.title | CELL | - |
dc.citation.volume | 176 | - |
dc.contributor.author | Rubin, Adam J. | - |
dc.contributor.author | Parker, Kevin R. | - |
dc.contributor.author | Satpathy, Ansuman T. | - |
dc.contributor.author | Qi, Yanyan | - |
dc.contributor.author | Wu, Beijing | - |
dc.contributor.author | Ong, Alvin J. | - |
dc.contributor.author | Mumbach, Maxwell R. | - |
dc.contributor.author | Ji, Andrew L. | - |
dc.contributor.author | Kim, Daniel S. | - |
dc.contributor.author | Cho, Seung Woo | - |
dc.contributor.author | Zarnegar, Brian J. | - |
dc.contributor.author | Greenleaf, William J. | - |
dc.contributor.author | Chang, Howard Y. | - |
dc.contributor.author | Khavari, Paul A. | - |
dc.date.accessioned | 2023-12-21T19:43:07Z | - |
dc.date.available | 2023-12-21T19:43:07Z | - |
dc.date.created | 2019-01-22 | - |
dc.date.issued | 2019-01 | - |
dc.description.abstract | Here, we present Perturb-ATAC, a method that combines multiplexed CRISPR interference or knockout with genome-wide chromatin accessibility profiling in single cells based on the simultaneous detection of CRISPR guide RNAs and open chromatin sites by assay of transposase-accessible chromatin with sequencing (ATAC-seq). We applied Perturb-ATAC to transcription factors (TFs), chromatin-modifying factors, and noncoding RNAs (ncRNAs) in ∼4,300 single cells, encompassing more than 63 genotype-phenotype relationships. Perturb-ATAC in human B lymphocytes uncovered regulators of chromatin accessibility, TF occupancy, and nucleosome positioning and identified a hierarchy of TFs that govern B cell state, variation, and disease-associated cis-regulatory elements. Perturb-ATAC in primary human epidermal cells revealed three sequential modules of cis-elements that specify keratinocyte fate. Combinatorial deletion of all pairs of these TFs uncovered their epistatic relationships and highlighted genomic co-localization as a basis for synergistic interactions. Thus, Perturb-ATAC is a powerful strategy to dissect gene regulatory networks in development and disease. | - |
dc.identifier.bibliographicCitation | CELL, v.176, no.1-2, pp.361 - 376 | - |
dc.identifier.doi | 10.1016/j.cell.2018.11.022 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.scopusid | 2-s2.0-85059878068 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25775 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0092867418315149?via%3Dihub | - |
dc.identifier.wosid | 000455410800030 | - |
dc.language | 영어 | - |
dc.publisher | CELL PRESS | - |
dc.title | Coupled Single-Cell CRISPR Screening and Epigenomic Profiling Reveals Causal Gene Regulatory Networks | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.description.journalRegisteredClass | scie | - |
dc.subject.keywordAuthor | ATAC-seq | - |
dc.subject.keywordAuthor | chromatin accessibility | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | epigenomics | - |
dc.subject.keywordAuthor | pooled screens | - |
dc.subject.keywordAuthor | single-cell genomics | - |
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