There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 3289 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 3283 | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 35 | - |
dc.contributor.author | Chung, Boram | - |
dc.contributor.author | Shin, Gi Won | - |
dc.contributor.author | Choi, Woong | - |
dc.contributor.author | Joo, Jinmyoung | - |
dc.contributor.author | Jeon, Sangmin | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2023-12-22T01:46:36Z | - |
dc.date.available | 2023-12-22T01:46:36Z | - |
dc.date.created | 2019-01-08 | - |
dc.date.issued | 2014-12 | - |
dc.description.abstract | Antibody-conjugated magnetic nanoparticles (Ab-MNPs) have potential in pathogen detection because they allow target cells to be easily separated from complex sample matrices. However, the sensitivity and specificity of pathogen capture by Ab-MNPs generally vary according to the types of MNPs, antibodies, and sample matrices, as well as preparation methods, including immobilization. Therefore, achieving a reproducible analysis utilizing Ab-MNPs as a pathogen detection method requires accurate characterization of Ab-MNP capture ability and standardization of all handling processes. In this study, we used high-resolution CE-single strand conformational polymorphism coupled with a stuffer-free multiplex ligation-dependent probe amplification system to characterize Ab-MNPs. The capture ability of Ab-MNPs targeting Salmonella enteritidis and nine pathogens, including S. enteritidis, was analyzed in phosphate buffer and milk. The effect of storage conditions on the stability of Ab-MNPs was also assessed. The results showed that the stuffer-free multiplex ligation-dependent probe amplification system has the potential to serve as a standard characterization method for Ab-MNPs. Moreover, the precise characterization of Ab-MNPs facilitated robust pathogen detection in various applications. | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.35, no.23, pp.3283 - 3289 | - |
dc.identifier.doi | 10.1002/elps.201400180 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.scopusid | 2-s2.0-84914095557 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25693 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201400180 | - |
dc.identifier.wosid | 000345972900001 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Precise characterization method of antibody-conjugated magnetic nanoparticles for pathogen detection using stuffer-free multiplex ligation-dependent probe amplification | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.