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Park, Sunghoon
Biochemical Engineering Lab.
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dc.citation.endPage 120 -
dc.citation.startPage 113 -
dc.citation.title METABOLIC ENGINEERING -
dc.citation.volume 47 - Seok, Joo Yeon - Yang, Jina - Choi, Sang Jin - Lim, Hyun Gyu - Choi, Un Jong - Kim, Kyung-Jin - Park, Sunghoon - Yoo, Tae Hyeon - Jung, Gyoo Yeol - 2023-12-21T20:45:22Z - 2023-12-21T20:45:22Z - 2018-05-21 - 2018-05 -
dc.description.abstract 3-Hydroxypropionic acid (3-HP) is an important platform chemical, and biological production of 3-HP from glycerol as a carbon source using glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) has been revealed to be effective because it involves a relatively simple metabolic pathway and exhibits higher yield and productivity than other biosynthetic pathways. Despite the successful attempts of 3-HP production from glycerol, the biological process suffers from problems arising from low activity and inactivation of the two enzymes. To apply the directed evolutionary approach to engineer the 3-HP production system, we constructed a synthetic selection device using a 3-HP-responsive transcription factor and developed a selection approach for screening 3-HP-producing microorganisms. The method was applied to an ALDH library, specifically aldehyde-binding site library of alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH). Only two serial cultures resulted in enrichment of strains showing increased 3-HP production, and an isolated KGSADH variant enzyme exhibited a 2.79-fold higher catalytic efficiency toward its aldehyde substrate than the wild-type one. This approach will provide the simple and efficient tool to engineer the pathway enzymes in metabolic engineering. -
dc.identifier.bibliographicCitation METABOLIC ENGINEERING, v.47, pp.113 - 120 -
dc.identifier.doi 10.1016/j.ymben.2018.03.009 -
dc.identifier.issn 1096-7176 -
dc.identifier.scopusid 2-s2.0-85044109129 -
dc.identifier.uri -
dc.identifier.url -
dc.identifier.wosid 000433423600012 -
dc.language 영어 -
dc.title Directed evolution of the 3-hydroxypropionic acid production pathway by engineering aldehyde dehydrogenase using a synthetic selection device -
dc.type Article -
dc.description.isOpenAccess FALSE -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor 3-hydroxypropionic acid -
dc.subject.keywordAuthor Directed evolution -
dc.subject.keywordAuthor Metabolic engineering -
dc.subject.keywordAuthor Screening -
dc.subject.keywordAuthor Synthetic biology -
dc.subject.keywordAuthor Transcription factor -
dc.subject.keywordPlus SACCHAROMYCES-CEREVISIAE -
dc.subject.keywordPlus GLYCEROL METABOLISM -
dc.subject.keywordPlus BIOSYNTHESIS -
dc.subject.keywordPlus FERMENTATION -
dc.subject.keywordPlus MUTAGENESIS -
dc.subject.keywordPlus BIOSENSORS -
dc.subject.keywordPlus SYSTEM -
dc.subject.keywordPlus FUTURE -


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