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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 328 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 322 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Kim, Yeon Hee | - |
dc.contributor.author | Kim, Young Hee | - |
dc.contributor.author | Kim, Jung Sun | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T10:15:53Z | - |
dc.date.available | 2023-12-22T10:15:53Z | - |
dc.date.created | 2017-03-04 | - |
dc.date.issued | 2005-07 | - |
dc.description.abstract | Acylhomoserine lactones (AHLs) are known to be the triggering molecules in the quorum sensing mechanism of many gram-negative bacteria. In order to detect AHL inhibitors that are potential biofilm inhibitors, a convenient and sensitive bioassay was developed based on the beta-galactosiclase activity (beta-GAL) of a recombinant Agrobacterium tumefaciens strain. A series of commercially available AHLs were tested for inducing beta-GAL at varying concentrations in agar-plate and liquid cultures of the reporter strain. All AHLs tested exhibited a concentration-dependent induction, and octanoyl homoserine lactone (OHL) showed the highest sensitivity with a detection limit of 0.1 nM in the liquid culture assay. When fimbrolide, a known quorum sensing inhibitor, was added, induction of beta-GAL by OHL was repressed. The repression at a constant OHL concentration was dependent on the fimbrolide concentration with the detection limit below 1 ppm, indicating that this assay is a sensitive method for screening AHL inhibitors. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.10, no.4, pp.322 - 328 | - |
dc.identifier.doi | 10.1007/BF02931849 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.scopusid | 2-s2.0-24344446265 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25377 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2FBF02931849 | - |
dc.identifier.wosid | 000231661500006 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Development of a sensitive bioassay method for quorum sensing inhibitor screening using a recombinant Agrobacterium tumefaciens | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | quorum sensing inhibitor | - |
dc.subject.keywordAuthor | bioassay | - |
dc.subject.keywordAuthor | acyl homoserine lactones | - |
dc.subject.keywordAuthor | Agrobacterium tumefaciens | - |
dc.subject.keywordAuthor | fimbrolide | - |
dc.subject.keywordAuthor | biofilm inhibitor | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | SIGNAL MOLECULES | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | BIOFILMS | - |
dc.subject.keywordPlus | COMMUNICATION | - |
dc.subject.keywordPlus | AUTOINDUCERS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | ANALOGS | - |
dc.subject.keywordPlus | PROTEIN | - |
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