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DC Field | Value | Language |
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dc.citation.endPage | 76 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 69 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 13 | - |
dc.contributor.author | Bae, Jong Wan | - |
dc.contributor.author | Shin, Seunghee | - |
dc.contributor.author | Raj, S. Mohan | - |
dc.contributor.author | Lee, Song Eun | - |
dc.contributor.author | Lee, Sun-Gu | - |
dc.contributor.author | Jeong, Yong-Joo | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T09:06:07Z | - |
dc.date.available | 2023-12-22T09:06:07Z | - |
dc.date.created | 2017-03-04 | - |
dc.date.issued | 2008-01 | - |
dc.description.abstract | A recombinant Escherichia coli (pBAB1) containing styrene monooxygenase (SMO) was developed for the conversion of styrene to enantiopure (S)-styrene oxide that is an important chiral building block in organic synthesis. The styAB genes encoding SMO was cloned into a multicopy plasmid under the tightly regulated promoter of bacterial L-arabinose operon which is inducible by L-arabinose. The recombinant showed that expression level of StyA protein and whole-cell SMO activities were varied depending on the concentration of the inducer L-arabinose. The maximum SMO activity was 108 U/g cdw when the cells were induced with 0.2% L-arabinose. SDS-PAGE and Western blot analyses indicated that whole-cell SMO activity was strongly correlated with the expression level of StyA protein. Organic-aqueous two-phase experiment could yield 50 mM enantiopure (S)-styrene oxide in organic phase in 18 h, but the recombinant SMO activity was unstable during the reaction. The expression of styAB under the control Of L-arabinose promoter was significantly repressed in the presence of glucose. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.13, no.1, pp.69 - 76 | - |
dc.identifier.doi | 10.1007/s12257-007-0172-z | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.scopusid | 2-s2.0-40549106156 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25364 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs12257-007-0172-z | - |
dc.identifier.wosid | 000253582400010 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Construction and characterization of a recombinant whole-cell biocatalyst of Escherichia coli expressing styrene monooxygenase under the control of arabinose promoter | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | (S)-styrene oxide | - |
dc.subject.keywordAuthor | styrene monooxygenase | - |
dc.subject.keywordAuthor | arabinose promoter | - |
dc.subject.keywordAuthor | pBAD | - |
dc.subject.keywordAuthor | whole-cell biocatalyst | - |
dc.subject.keywordPlus | PSEUDOMONAS-PUTIDA SN1 | - |
dc.subject.keywordPlus | (S)-STYRENE OXIDE | - |
dc.subject.keywordPlus | ARAC PROTEIN | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | CULTURES | - |
dc.subject.keywordPlus | GENES | - |
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