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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 367 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 362 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 19 | - |
dc.contributor.author | Yeo, Yun-Ji | - |
dc.contributor.author | Shin, Seunghee | - |
dc.contributor.author | Lee, Sun-Gu | - |
dc.contributor.author | Park, Sunghoon | - |
dc.contributor.author | Jeong, Yong-Joo | - |
dc.date.accessioned | 2023-12-22T08:07:18Z | - |
dc.date.available | 2023-12-22T08:07:18Z | - |
dc.date.created | 2017-03-04 | - |
dc.date.issued | 2009-04 | - |
dc.description.abstract | In recombinant strains, many proteins and enzymes are expressed as inactive and insoluble inclusion bodies. For soluble expression of an active form of StyB, an NADH-flavin oxidoreductase, several recombinant Escherichia coli strains were developed and tested. Among them, strain BL21(DE3)pLysS effectively produced an active and soluble form of StyB as about 9% of the total protein content, when cultivated at 20 degrees C with 0.5 mM IPTC. The solubly expressed Styli has the highest oxidoreductase activity at pH 6.5-7.5 and 37 degrees C. Substrate dependence profiles of the StyB-catalyzed reaction showed that the maximum specific activity (V(m)) and half saturation constant (K(m)) were 1,867 +/- 148 U/mg protein and 51.6 +/- 11 mu M for NADH, and 1,274 +/- 34 U/mg protein and 8.2 +/- 1.2 mu M for FAD, respectively. This indicates that solubly produced StyB has 6- to 9-fold higher oxidoreductase activities than the in vitro refolded StyB from inclusion bodies. | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.4, pp.362 - 367 | - |
dc.identifier.doi | 10.4014/jmb.0806.382 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.scopusid | 2-s2.0-67649321851 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25352 | - |
dc.identifier.url | http://www.jmb.or.kr/journal/viewJournal.html?year=2009&vol=19&num=4&page=362 | - |
dc.identifier.wosid | 000265574100005 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Production, Purification, and Characterization of Soluble NADH-Flavin Oxidoreductase (StyB) from Pseudomonas putida SN1 | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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