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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 657 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 649 | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 84 | - |
dc.contributor.author | Raj, Subramanian Mohan | - |
dc.contributor.author | Rathnasingh, Chelladurai | - |
dc.contributor.author | Jung, Woo-Chel | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T07:39:58Z | - |
dc.date.available | 2023-12-22T07:39:58Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2009-09 | - |
dc.description.abstract | The top-valued platform chemical, 3-hydroxypropionic acid (3-HP), has a wide range of industrial applications but its biological production is not well established. Previously, the production of 3-HP from glycerol was demonstrated using a recombinant Escherichia coli strain expressing glycerol dehydratase (dhaB) and aldehyde dehydrogenase (aldH). The present investigation focuses on the effect of the culture conditions on the production of 3-HP from glycerol. The physicochemical parameters, such as pH, IPTG concentration, liquid-to-flask volume ratio, and substrate concentration, were examined in flask-scale experiments and obtained the highest titer of 3-HP at 4.4 g l(-1) in 48 h. When a fed-batch process was carried out in a bioreactor under pH-regulated conditions, the recombinant E. coli produced 3-HP at 31 g l(-1) in 72 h with a yield of 0.35 mol mol(-1) glycerol. The maximum specific rate of 3-HP production was estimated to be 3.41 mmol g(-1) cdw h(-1) between 12 and 24 h. Other than 3-HP, propionic acid (3.4 g l(-1)), 1,3-propanediol (2.4 g l(-1)), and lactic acid (1.6 g l(-1)) were produced as the major by-products. This paper reports for the first time a commercially meaningful high titer of 3-HP production. | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.84, no.4, pp.649 - 657 | - |
dc.identifier.doi | 10.1007/s00253-009-1986-8 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.scopusid | 2-s2.0-69949118104 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25349 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00253-009-1986-8 | - |
dc.identifier.wosid | 000269911000006 | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER | - |
dc.title | Effect of process parameters on 3-hydroxypropionic acid production from glycerol using a recombinant Escherichia coli | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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