There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1265 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1253 | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 90 | - |
dc.contributor.author | Ashok, Somasundar | - |
dc.contributor.author | Raj, Subramanian Mohan | - |
dc.contributor.author | Rathnasingh, Chelladurai | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T06:10:17Z | - |
dc.date.available | 2023-12-22T06:10:17Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2011-05 | - |
dc.description.abstract | Klebsiella pneumoniae converts glycerol to the specialty chemical 1,3-propanediol (1,3-PDO), which is used for the production of polytrimethylene terepthalate (PTT). In this study, an NAD(+)-dependent gamma-glutamyl-gammaaminobutyraldehyde dehydrogenase (PuuC) of K. pneumoniae DSM 2026, which oxidizes 3-hydroxypropionaldehyde to a platform chemical 3-hydroxypropionic acid (3-HP), was cloned and overexpressed in K. pneumoniae DSM 2026 for the co-production of 3-HP and 1,3-PDO from glycerol. In addition, the gene dhaT, encoding NADH-dependent 1,3-propanediol oxidoreductase (1,3-PDOR), was deleted from the chromosome for the balanced production of 3-HP and 1,3-PDO. The recombinant K. pneumoniae Delta dhaT, expressing puuC, produced 3.6 g 3-HP and 3.0 g 1,3-PDO per liter with an average yield of 81% on glycerol carbon in shake flask culture under microaerobic conditions. When a fedbatch culture was carried out under microaerobic conditions at pH 7.0 in a 5-1 bioreactor, the recombinant K. pneumoniae Delta dhaT (puuC) strain produced 16.0 g 3-HP and 16.8 g 1,3-PDO per liter with a cumulative yield of 51% on glycerol carbon in 24 h. The production of 1,3PDO in the dhaT-deletion mutant was attributed to the expression of NAD(P) H-dependent hypothetical oxidore-ductase. This study demonstrates the feasibility of obtaining two commercially valuable chemicals, 3-HP and 1,3PDO, at a significant scale | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.90, no.4, pp.1253 - 1265 | - |
dc.identifier.doi | 10.1007/s00253-011-3148-z | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.scopusid | 2-s2.0-79955564954 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25343 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00253-011-3148-z | - |
dc.identifier.wosid | 000290574700006 | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER | - |
dc.title | Development of recombinant Klebsiella pneumoniae Delta dhaT strain for the co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.