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DC Field | Value | Language |
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dc.citation.endPage | 640 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 633 | - |
dc.citation.title | JOURNAL OF BIOTECHNOLOGY | - |
dc.citation.volume | 157 | - |
dc.contributor.author | Rathnasingh, Chelladurai | - |
dc.contributor.author | Raj, Subramanian Mohan | - |
dc.contributor.author | Lee, Youjin | - |
dc.contributor.author | Catherine, Christy | - |
dc.contributor.author | Ashoka, Somasundar | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T05:18:44Z | - |
dc.date.available | 2023-12-22T05:18:44Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2012-02 | - |
dc.description.abstract | Malonyl-CoA is an intermediary compound that is produced during fatty acid metabolism. Our study aimed to produce the commercially important platform chemical 3-hydroxypropionic acid (3-HP) from its immediate precursor malonyl-CoA by recombinant Escherichia coli strains heterologously expressing the mcr gene of Chloroflexus aurantiacus DSM 635, encoding an NADPH-dependent malonyl-CoA reductase (MCR). The recombinant E. coli overexpressing mcr under the 15 promoter showed MCR activity of 0.015 U mg(-1) protein in crude cell extract and produced 0.71 mmol/L of 3-HP in 24 h in shake flask cultivation under aerobic conditions with glucose as the sole source of carbon. When acetyl-CoA carboxylase and biotinilase, encoded by the genes accADBCb (ACC) of E. coli K-12 were overexpressed along with MCR, the final 3-HP titer improved by 2-fold, which is 1.6 mM. Additional expression of the gene pntAB, encoding nicotinamide nucleotide transhydrogenase that converts NADH to NADPH, increased 3-HP production to 2.14 mM. The strain was further developed by deleting the sucAB gene, encoding a-ketoglutarate dehydrogenase complex in tricarboxylic acid (TCA) cycle, or blocking lactate and acetate production pathways, and evaluated for the production of 3-HP. We report on the feasibility of producing 3-HP from glucose through the malonyl-CoA pathway. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOTECHNOLOGY, v.157, no.4, pp.633 - 640 | - |
dc.identifier.doi | 10.1016/j.jbiotec.2011.06.008 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.scopusid | 2-s2.0-84857449564 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25337 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0168165611003075 | - |
dc.identifier.wosid | 000301900900025 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant Escherichia coli strains | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 3-hydroxypropionic acid | - |
dc.subject.keywordAuthor | Malonyl-CoA | - |
dc.subject.keywordAuthor | Malonyl-CoA reductase | - |
dc.subject.keywordAuthor | Acetyl-CoA carboxylase | - |
dc.subject.keywordAuthor | Transhydrogenase | - |
dc.subject.keywordAuthor | Chloroflexus aurantiacus | - |
dc.subject.keywordPlus | ACETYL-COA | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | CARBOXYLASE | - |
dc.subject.keywordPlus | COENZYME | - |
dc.subject.keywordPlus | FIXATION | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | GENES | - |
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