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DC Field | Value | Language |
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dc.citation.endPage | 24 | - |
dc.citation.startPage | 10 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 15 | - |
dc.contributor.author | Ashok, Somasundar | - |
dc.contributor.author | Raj, Subramanian Mohan | - |
dc.contributor.author | Ko, Yeounjoo | - |
dc.contributor.author | Sankaranarayanan, Mugesh | - |
dc.contributor.author | Zhou, Shengfang | - |
dc.contributor.author | Kumar, Vinod | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T04:14:39Z | - |
dc.date.available | 2023-12-22T04:14:39Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2013-01 | - |
dc.description.abstract | 3-Hydroxypropionic acid (3-HP), an industrially important platform chemical, is used as a precursor during the production of many commercially important chemicals. Recently, recombinant strains of K. pneumoniae overexpressing an NAD(+)-dependent gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (PuuC) enzyme of K. pneumoniae DSM 2026 were shown to produce 3-HP from glycerol without the addition coenzyme B-12, which is expensive. However, 3-HP production in K. pneumoniae is accompanied with NADH generation, and this always results in large accumulation of 1,3-propanediol (1,3-PDO) and lactic acid. In this study, we investigated the potential use of nitrate as an electron acceptor both to regenerate NAD(+) and to prevent the formation of byproducts during anaerobic production of 3-HP from glycerol. Nitrate addition could improve NAD(+) regeneration, but decreased glycerol flux towards 3-HP production. To divert more glycerol towards 3-HP, a novel recombinant strain K. pneumoniae Delta glpK Delta dhaT (puuC) was developed by disrupting the glpK gene, which encodes glycerol kinase, and the dhaT gene, which encodes 1,3-propanediol oxidoreductase. This strain showed improved cellular NAD(+) concentrations and a high carbon flux towards 3-HP production. Through anaerobic cultivation in the presence of nitrate, this recombinant strain produced more than 40 +/- 3 mM 3-HP with more than 50% yield on glycerol in shake flasks and 250 +/- 10 mM 3-HP with approximately 30% yield on glycerol in a fed-batch bioreactor. | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.15, pp.10 - 24 | - |
dc.identifier.doi | 10.1016/j.ymben.2012.09.004 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25331 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1096717612000997 | - |
dc.identifier.wosid | 000313882500002 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Effect of puuC overexpression and nitrate addition on glycerol metabolism and anaerobic 3-hydroxypropionic acid production in recombinant Klebsiella pneumoniae Delta glpK Delta dhaT | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae | - |
dc.subject.keywordAuthor | 3-hydroxypropionic acid | - |
dc.subject.keywordAuthor | Glycerol metabolism | - |
dc.subject.keywordAuthor | Nitrate | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | UTILIZES 3-HYDROXYPROPIONALDEHYDE | - |
dc.subject.keywordPlus | ALDEHYDE DEHYDROGENASE | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | REDUCTASE | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | NITRITE | - |
dc.subject.keywordPlus | DISSIMILATION | - |
dc.subject.keywordPlus | RESPIRATION | - |
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