There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 51 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 43 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 18 | - |
dc.contributor.author | Arasu, Mariadhas Valan | - |
dc.contributor.author | Sarkar, Ritam | - |
dc.contributor.author | Sekar, Balaji Sundara | - |
dc.contributor.author | Kumar, Vinod | - |
dc.contributor.author | Rathnasingh, Chelladurai | - |
dc.contributor.author | Choi, Jin-dal-rae | - |
dc.contributor.author | Song, Hyohak | - |
dc.contributor.author | Seung, Doyoung | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T04:11:49Z | - |
dc.date.available | 2023-12-22T04:11:49Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2013-02 | - |
dc.description.abstract | Vitamin B-12 is a complex biomolecule that acts as a cofactor for a variety of enzymes in microbial metabolism. Pseudomonas denitrificans is exclusively used as an industrial strain for the production of vitamin B-12 under aerobic conditions. However, only a few strains of Pseudomonas have been reported to possess the capability of producing this vitamin and they are strongly patent-protected. To improve the applicability of the vitamin B-12-producing microorganisms, a new isolate was obtained from municipal waste samples and characterized for its biological properties. The new isolate, designated as SP2, was identified to be a Pseudomonas species based on the sequence homology of its 16S rDNA. Pseudomonas species SP2 had essential genes for vitamin B-12 synthesis such as cobB and cobQ and produced a similar amount of vitamin B-12 (10.6 +/- 0.05 mu g/mL) as P. denitrificans ATCC 13867 in 24 h flask culture. SP2 grew well under aerobic condition with the maximum specific growth rate (A mu (max) ) of 0.91 +/- 0.03/h, but showed a poor growth under micro-aerobic conditions. SP2 was resistant to antibiotics like streptomycin, carbenicillin, ampicillin, cefpodoxime, colistin, nalidixic acid and sparfloxacin. The ability of SP2 to grow faster and produce vitamin B-12 under aerobic conditions makes it a promising host for the production of some biochemicals requiring a coenzyme B-12-dependent enzyme, such as glycerol dehydratase. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.18, no.1, pp.43 - 51 | - |
dc.identifier.doi | 10.1007/s12257-012-0518-z | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.scopusid | 2-s2.0-84874826165 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25330 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs12257-012-0518-z | - |
dc.identifier.wosid | 000316076600006 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Isolation of a novel Pseudomonas species SP2 producing vitamin B-12 under aerobic condition | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | vitamin B-12 | - |
dc.subject.keywordAuthor | Pseudomonas species SP2 | - |
dc.subject.keywordAuthor | Pseudomonas denitrificans ATCC 13867 | - |
dc.subject.keywordAuthor | 3-hydroxypropionic acid | - |
dc.subject.keywordPlus | DENITRIFICANS | - |
dc.subject.keywordPlus | AERUGINOSA | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordPlus | ACID | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.