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DC Field | Value | Language |
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dc.citation.number | 5 | - |
dc.citation.startPage | e62666 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 8 | - |
dc.contributor.author | Zhou, Shengfang | - |
dc.contributor.author | Raj, Subramanian Mohan | - |
dc.contributor.author | Ashok, Somasundar | - |
dc.contributor.author | Edwardraja, Selvakumar | - |
dc.contributor.author | Lee, Sun-gu | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T04:06:24Z | - |
dc.date.available | 2023-12-22T04:06:24Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2013-05 | - |
dc.description.abstract | The gene encoding an NAD+-dependent, 3-hydroxyisobutyrate dehydrogenase (3HIBDH-IV) from Pseudomonas denitrificans ATCC 13867 was cloned and expressed in Escherichia coli BL 21 (DE3) and characterized to understand its physiological relevance in the degradation of 3-hydroxypropionic acid (3-HP). The deduced amino acid sequence showed high similarity to other 3-hydroxyisobutyrate dehydrogenase isozymes (3HIBDHs) of P. denitrificans ATCC 13867. A comparison of 3HIBDH-IV with its relevant enzymes along with molecular docking studies suggested that Lys171, Asn175 and Gly123 are important for its catalytic function on 3-hydroxyacids. The recombinant 3HIBDH-IV was purified to homogeneity utilizing a Ni-NTA-HP resin column in high yield. 3HIBDH-IV was very specific to (S)-3-hydroxyisobutyrate, but also catalyzed the oxidation of 3-HP to malonate semialdehyde. The specific activity and half-saturation constant (K-m) for 3-HP at 30 degrees C and pH 9.0 were determined to be 17 U/mg protein and 1.0 mM, respectively. Heavy metals, such as Ag+ and Hg2+, completely inhibited the 3HIBDH-IV activity, whereas dithiothreitol, 2-mercaptoethanol and ethylenediaminetetraacetic acid increased its activity 1.5-1.8-fold. This paper reports the characteristics of 3HIBDH-IV as well as its probable role in 3-HP degradation | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.8, no.5, pp.e62666 | - |
dc.identifier.doi | 10.1371/journal.pone.0062666 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.scopusid | 2-s2.0-84877027359 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25327 | - |
dc.identifier.url | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062666 | - |
dc.identifier.wosid | 000319167000069 | - |
dc.language | 영어 | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Cloning, Expression and Characterization of 3-Hydroxyisobutyrate Dehydrogenase from Pseudomonas denitrificans ATCC 13867 | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | BETA-HYDROXYACID DEHYDROGENASES | - |
dc.subject.keywordPlus | AUTOTROPHIC CO2 FIXATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | 3-HYDROXYPROPIONIC ACID | - |
dc.subject.keywordPlus | PUTIDA E23 | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | PRODUCT | - |
dc.subject.keywordPlus | GENE | - |
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