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DC Field | Value | Language |
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dc.citation.endPage | 3187 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 3177 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 110 | - |
dc.contributor.author | Zhou, Shengfang | - |
dc.contributor.author | Catherine, Christy | - |
dc.contributor.author | Rathnasingh, Chelladurai | - |
dc.contributor.author | Somasundar, Ashok | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T03:10:25Z | - |
dc.date.available | 2023-12-22T03:10:25Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2013-12 | - |
dc.description.abstract | 3-Hydroxypropionic acid (3-HP) can be produced from glycerol through two sequential enzymatic reactions that are catalyzed by a coenzyme B-12-dependent glycerol dehydratase and an NAD(P)(+)-dependent aldehyde dehydrogenase (ALDH), respectively. Pseudomonas denitrificans synthesizes coenzyme B-12 under aerobic conditions, where NAD(P)(+) is regenerated efficiently. Hence, it is considered an ideal host for the production of 3-HP from glycerol under aerobic conditions. In this study, recombinant strains of P. denitrificans were developed and their potential for the production of 3-HP from glycerol was evaluated. When the enzymes, glycerol dehydratase (DhaB) and glycerol dehydratase reactivase (GdrAB), of Klebsiella pneumoniae were expressed heterologously, P. denitrificans could produce 3-HP at 37.7mmol/L with 62% (mol/mol) yield on glycerol. Glucose was required as the carbon and energy sources for cell growth. The overexpression of heterologous ALDH was not essential; however, the titer and yield of 3-HP were improved to 54.7mmol/L and 67% (mol/mol), respectively, when an ALDH gene (puuC) from K. pneumoniae was overexpressed. One serious drawback hindering the use of P. denitrificans as a recombinant host for 3-HP production is that it oxidizes 3-HP to malonate and utilizes 3-HP as a carbon source for growth. This is the first report on the development and use of recombinant P. denitrificans for 3-HP production from glycerol. Biotechnol. Bioeng. 2013;110: 3177-3187. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.110, no.12, pp.3177 - 3187 | - |
dc.identifier.doi | 10.1002/bit.24980 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.scopusid | 2-s2.0-84886244943 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25322 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/bit.24980/abstract | - |
dc.identifier.wosid | 000328136700012 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Production of 3-Hydroxypropionic Acid From Glycerol by Recombinant Pseudomonas denitrificans | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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