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DC Field | Value | Language |
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dc.citation.endPage | 4398 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 4389 | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 98 | - |
dc.contributor.author | Zhou, Shengfang | - |
dc.contributor.author | Ashok, Somasundar | - |
dc.contributor.author | Ko, Yeounjoo | - |
dc.contributor.author | Kim, Dong-Myung | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T02:40:08Z | - |
dc.date.available | 2023-12-22T02:40:08Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2014-05 | - |
dc.description.abstract | Pseudomonas denitrificans is a gram-negative bacterium that can produce vitamin B-12 under aerobic conditions. Recently, recombinant strains of P. denitrificans overexpressing a vitamin B-12-dependent glycerol dehydratase (DhaB) were developed to produce 3-hydroxypropionic acid (3-HP) from glycerol. The recombinant P. denitrificans could produce 3-HP successfully under aerobic conditions without an exogenous supply of vitamin B-12, but the 3-HP produced disappeared during extended cultivation due to the 3-HP degradation activity in this strain. This study developed mutant strains of P. denitrificans that do not degrade 3-HP. The following eight candidate enzymes, which might be responsible for 3-HP degradation, were selected, cloned, and studied for their activity in Escherichia coli: four (putative) 3-hydroxyisobutyrate dehydrogenases (3HIBDH), a putative 3-HP dehydrogenase (3HPDH), an alcohol dehydrogenase (ADH), and two choline dehydrogenases (CHDH). Among them, 3HIBDHI, 3HIBDHIV, and 3HPDH exhibited 3-HP degrading activity when expressed heterologously in E. coli. When 3hpdh alone or along with 3hibdhIV were disrupted from P. denitrificans, the mutant P. denitrificans exhibited greatly reduced 3-HP degradation activity that could not grow on 3-HP as the sole carbon and energy source. When the double mutant P. denitrificans Delta 3hpdh Delta 3hibdhIV was transformed with DhaB, an improved 3-HP yield (0.78 mol/mol) compared to that of the wild-type counterpart (0.45 mol/mol) was obtained from a 24-h flask culture. This study indicates that 3hpdh and 3hibdhIV (to a lesser extent) are mainly responsible for 3-HP degradation in P. denitrificans and their deletion can prevent 3-HP degradation during its production by recombinant P. denitrificans. | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.98, no.10, pp.4389 - 4398 | - |
dc.identifier.doi | 10.1007/s00253-014-5562-5 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.scopusid | 2-s2.0-84900817777 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25318 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00253-014-5562-5 | - |
dc.identifier.wosid | 000335460700010 | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER | - |
dc.title | Development of a deletion mutant of Pseudomonas denitrificans that does not degrade 3-hydroxypropionic acid | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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