There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 10425 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 10417 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | - |
dc.citation.volume | 39 | - |
dc.contributor.author | Ainala, Satish Kumar | - |
dc.contributor.author | Seol, Eunhee | - |
dc.contributor.author | Sekar, Balaji Sundara | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T02:36:27Z | - |
dc.date.available | 2023-12-22T02:36:27Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2014-07 | - |
dc.description.abstract | Citrobacter amalonaticus Y19 (Y19) can produce hydrogen (H-2) from oxidation of carbon monoxide (CO) via the water-gas shift reaction. The reaction is catalyzed by two enzymes, carbon monoxide dehydrogenase (CODH) and carbon monoxide-dependent hydrogenase (CO-Hyd). The contig genome sequencing of Y19 exhibited the presence of unique CO oxidizing gene clusters encoding CODH (cooFS), CO-Hyd (cooMKLXUH) and a putative CO-responsive transcriptional activator (cooA). To improve CO-dependent H-2 production activity, we developed recombinant Y19 by homologously over-expressing cooA. The overexpression of cooA improved the whole-cell CO-dependent H-2 production activity (3.4-fold), and enzyme activities of CODH (5.3-fold) and CO-Hyd (1.2-fold). Furthermore, quantitative PCR analysis revealed a significant increase in the transcription of the genes located in CODH and CO-Hyd operons of recombinant Y19. The high CO-dependent H-2 production activity of the recombinant C. amalonaticus was stably maintained during repeated exposure to CO. | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.39, no.20, pp.10417 - 10425 | - |
dc.identifier.doi | 10.1016/j.ijhydene.2014.04.148 | - |
dc.identifier.issn | 0360-3199 | - |
dc.identifier.scopusid | 2-s2.0-84902535587 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25317 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0360319914012063 | - |
dc.identifier.wosid | 000338388200010 | - |
dc.language | 영어 | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Improvement of carbon monoxide-dependent hydrogen production activity in Citrobacter amalonaticus Y19 by over-expressing the CO-sensing transcriptional activator, CooA | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.