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DC Field | Value | Language |
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dc.citation.endPage | 1535 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1526 | - |
dc.citation.title | BIOTECHNOLOGY JOURNAL | - |
dc.citation.volume | 9 | - |
dc.contributor.author | Ko, Yeounjoo | - |
dc.contributor.author | Ashok, Somasundar | - |
dc.contributor.author | Ainala, Satish Kumar | - |
dc.contributor.author | Sankaranarayanan, Mugesh | - |
dc.contributor.author | Chun, Ah Yeong | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T01:46:58Z | - |
dc.date.available | 2023-12-22T01:46:58Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2014-12 | - |
dc.description.abstract | Coenzyme B-12 (Vitamin B-12) is one of the most complex biomolecules and an essential cofactor required for the catalytic activity of many enzymes. Pseudomonas denitrificans synthesizes coenzyme B-12 in an oxygen-dependent manner using a pathway encoded by more than 25 genes that are located in six different operons. Escherichia coli, a robust and suitable host for metabolic engineering was used to produce coenzyme B-12. These genes were cloned into three compatible plasmids and expressed heterologously in E. coli BL21 (DE3). Real-time PCR, SDS-PAGE analysis and bioassay showed that the recombinant E. coli expressed the coenzyme B-12 synthetic genes and successfully produced coenzyme B-12. However, according to the quantitative determination by inductively coupled plasma-mass spectrometry, the amount of coenzyme B-12 produced by the recombinant E. coli (0.21 +/- 0.02 mu g/g cdw) was approximately 13-fold lower than that by P. denitrificans (2.75 +/- 0.22 mu g/g cdw). Optimization of the culture conditions to improve the production of coenzyme B-12 by the recombinant E. coli was successful, and the highest titer (0.65 +/- 0.03 mu g/g cdw) of coenzyme B-12 was obtained. Interestingly, although the synthesis of coenzyme B-12 in P. denitrificans is strictly oxygen-dependent, the recombinant E. coli could produce coenzyme B-12 under anaerobic conditions. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY JOURNAL, v.9, no.12, pp.1526 - 1535 | - |
dc.identifier.doi | 10.1002/biot.201400221 | - |
dc.identifier.issn | 1860-6768 | - |
dc.identifier.scopusid | 2-s2.0-84925933289 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25311 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/biot.201400221/abstract | - |
dc.identifier.wosid | 000346021200013 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Coenzyme B-12 can be produced by engineered Escherichia coli under both anaerobic and aerobic conditions | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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