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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 7355 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 7348 | - |
dc.citation.title | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.citation.volume | 80 | - |
dc.contributor.author | Zhang, Yan | - |
dc.contributor.author | Pan, Jiang | - |
dc.contributor.author | Luan, Zheng-Jiao | - |
dc.contributor.author | Xu, Guo-Chao | - |
dc.contributor.author | Park, Sunghoon | - |
dc.contributor.author | Xu, Jian-He | - |
dc.date.accessioned | 2023-12-22T01:46:56Z | - |
dc.date.available | 2023-12-22T01:46:56Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2014-12 | - |
dc.description.abstract | A novel nonheme chloroperoxidase (RhEst1), with promiscuous esterase activity for enantioselective hydrolysis of ethyl (S)-2,2-dimethylcyclopropanecarboxylate, was identified from a shotgun library of Rhodococcus sp. strain ECU1013. RhEst1 was overexpressed in Escherichia coli BL21(DE3), purified to homogeneity, and functionally characterized. Fingerprinting analysis revealed that RhEst1 prefers para-nitrophenyl (pNP) esters of short-chain acyl groups. pNP esters with a cyclic acyl moiety, especially that with a cyclobutanyl group, were also substrates for RhEst1. The K-m values for methyl 2,2-dimethylcyclopropanecarboxylate (DmCpCm) and ethyl 2,2-dimethylcyclopropane carboxylate (DmCpCe) were 0.25 and 0.43 mM, respectively. RhEst1 could serve as an efficient hydrolase for the bioproduction of optically pure (S)-2,2-dimethyl cyclopropane carboxylic acid (DmCpCa), which is an important chiral building block for cilastatin. As much as 0.5 M DmCpCe was enantioselectively hydrolyzed into (S)-DmCpCa, with a molar yield of 47.8% and an enantiomeric excess (ee) of 97.5%, indicating an extremely high enantioselectivity (E = 240) of this novel and unique biocatalyst for green manufacturing of highly valuable chiral chemicals. | - |
dc.identifier.bibliographicCitation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.80, no.23, pp.7348 - 7355 | - |
dc.identifier.doi | 10.1128/AEM.01597-14 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.scopusid | 2-s2.0-84910152264 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25310 | - |
dc.identifier.url | http://aem.asm.org/content/80/23/7348 | - |
dc.identifier.wosid | 000344163700025 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Cloning and Characterization of a Novel Esterase from Rhodococcus sp. for Highly Enantioselective Synthesis of a Chiral Cilastatin Precursor | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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