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DC Field | Value | Language |
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dc.citation.startPage | 4 | - |
dc.citation.title | BMC BIOLOGY | - |
dc.citation.volume | 12 | - |
dc.contributor.author | Cho, Byung-Kwan | - |
dc.contributor.author | Kim, Donghyuk | - |
dc.contributor.author | Knight, Eric M. | - |
dc.contributor.author | Zengler, Karsten | - |
dc.contributor.author | Palsson, Bernhard O. | - |
dc.date.accessioned | 2023-12-22T03:07:53Z | - |
dc.date.available | 2023-12-22T03:07:53Z | - |
dc.date.created | 2018-07-04 | - |
dc.date.issued | 2014-01 | - |
dc.description.abstract | Background: At the beginning of the transcription process, the RNA polymerase (RNAP) core enzyme requires a sigma-factor to recognize the genomic location at which the process initiates. Although the crucial role of sigma-factors has long been appreciated and characterized for many individual promoters, we do not yet have a genome-scale assessment of their function. Results: Using multiple genome-scale measurements, we elucidated the network of s-factor and promoter interactions in Escherichia coli. The reconstructed network includes 4,724 sigma-factor-specific promoters corresponding to transcription units (TUs), representing an increase of more than 300% over what has been previously reported. The reconstructed network was used to investigate competition between alternative sigma-factors (the sigma(70) and sigma(38) regulons), confirming the competition model of sigma substitution and negative regulation by alternative s-factors. Comparison with sigma-factor binding in Klebsiella pneumoniae showed that transcriptional regulation of conserved genes in closely related species is unexpectedly divergent. Conclusions: The reconstructed network reveals the regulatory complexity of the promoter architecture in prokaryotic genomes, and opens a path to the direct determination of the systems biology of their transcriptional regulatory networks. | - |
dc.identifier.bibliographicCitation | BMC BIOLOGY, v.12, pp.4 | - |
dc.identifier.doi | 10.1186/1741-7007-12-4 | - |
dc.identifier.issn | 1741-7007 | - |
dc.identifier.scopusid | 2-s2.0-84892777715 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/24288 | - |
dc.identifier.url | https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-12-4 | - |
dc.identifier.wosid | 000333128100001 | - |
dc.language | 영어 | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Genome-scale reconstruction of the sigma factor network in Escherichia coli: topology and functional states | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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