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DC Field | Value | Language |
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dc.citation.endPage | 333 | - |
dc.citation.startPage | 323 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 47 | - |
dc.contributor.author | Anh Duc Nguyen | - |
dc.contributor.author | Hwang, In Yeub | - |
dc.contributor.author | Lee, Ok Kyung | - |
dc.contributor.author | Kim, Donghyuk | - |
dc.contributor.author | Kalyuzhnaya, Marina G. | - |
dc.contributor.author | Mariyana, Rina | - |
dc.contributor.author | Hadiyati, Susila | - |
dc.contributor.author | Kim, Min Sik | - |
dc.contributor.author | Lee, Eun Yeol | - |
dc.date.accessioned | 2023-12-21T20:44:03Z | - |
dc.date.available | 2023-12-21T20:44:03Z | - |
dc.date.created | 2018-07-04 | - |
dc.date.issued | 2018-05 | - |
dc.description.abstract | Methane is considered a next-generation feedstock, and methanotrophic cell-based biorefinery is attractive for production of a variety of high-value compounds from methane. In this work, we have metabolically engineered Methylomicrobium alcaliphilum 20Z for 2,3-butanediol (2,3-BDO) production from methane. The engineered strain 20Z/pBudK.p, harboring the 2,3-BDO synthesis gene cluster (budABC) from Klebsiella pneumoniae, accumulated 2,3-BDO in methane-fed shake flask cultures with a titer of 35.66 mg/L. Expression of the most efficient gene cluster was optimized using selection of promoters, translation initiation rates (TIR), and the combination of 2,3-BDO synthesis genes from different sources. A higher 2,3-BDO titer of 57.7 mg/L was measured in the 20Z/pNBM-Re strain with budA of K. pneumoniae and budB of Bacillus subtilis under the control of the Tac promoter. The genome-scale metabolic network reconstruction of M. alcaliphilum 20Z enabled in silico gene knockout predictions using an evolutionary programming method to couple growth and 2,3-BDO production. The ldh, ack, and mdh genes in M. alcaliphilum 20Z were identified as potential knockout targets. Pursuing these targets, a triple-mutant strain Delta ldh Delta ack Delta mdh was constructed, resulting in a further increase of the 2,3-BDO titer to 68.8 mg/L. The productivity of this optimized strain was then tested in a fed-batch stirred tank bioreactor, where final product concentrations of up to 86.2 mg/L with a yield of 0.0318 g-(2,3-BDO) /g-CH4 were obtained under O-2-limited conditions. This study first demonstrates the strategy of in silico simulation-guided metabolic engineering and represents a proof-of-concept for the production of value-added compounds using systematic approaches from engineered methanotrophs. | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.47, pp.323 - 333 | - |
dc.identifier.doi | 10.1016/j.ymben.2018.04.010 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.scopusid | 2-s2.0-85046038397 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/24266 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1096717618301101?via%3Dihub | - |
dc.identifier.wosid | 000433423600032 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Systematic metabolic engineering of Methylomicrobium alcaliphilum 20Z for 2,3-butanediol production from methane | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Methanotrophic bacteria | - |
dc.subject.keywordAuthor | Glycolysis-based methane assimilation pathway | - |
dc.subject.keywordAuthor | Genome-scale models | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | 2,3-Butanediol | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE | - |
dc.subject.keywordPlus | GENETIC MANIPULATION | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | METHANOTROPHS | - |
dc.subject.keywordPlus | BIOCONVERSION | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | ACETOIN | - |
dc.subject.keywordPlus | YIELD | - |
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