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DC Field | Value | Language |
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dc.citation.endPage | 1329 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1320 | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 18 | - |
dc.contributor.author | Kim, Chi-Ju | - |
dc.contributor.author | Park, Juhee | - |
dc.contributor.author | Sunkara, Vijaya | - |
dc.contributor.author | Kim, Tae-Hyeong | - |
dc.contributor.author | Lee, Yongjin | - |
dc.contributor.author | Lee, Kyusang | - |
dc.contributor.author | Kim, Mi-Hyun | - |
dc.contributor.author | Cho, Yoon-Kyoung | - |
dc.date.accessioned | 2023-12-21T20:45:41Z | - |
dc.date.available | 2023-12-21T20:45:41Z | - |
dc.date.created | 2018-05-18 | - |
dc.date.issued | 2018-05 | - |
dc.description.abstract | The potential utility of circulating tumour DNA (ctDNA) in patient blood for cancer diagnostics and real-time monitoring of disease progression is highly recognized. However, the lack of automated and efficient methods for cell-free DNA (cfDNA) isolation from peripheral blood has remained a challenge for broader acceptance of liquid biopsy in general clinical settings. Here, we demonstrate a lab-on-a-disc system equipped with newly developed, electromagnetically actuated, and reversible diaphragm valves that allows fully automated and rapid (<30 min) isolation of cfDNA from whole blood (>3 ml) to achieve high detection sensitivity by minimizing the degradation of fragile ctDNA as well as contamination of wild-type DNA from abundant blood cells. As a proof of concept study, we used the lab-on-a-disc to isolate cfDNA from patients with non-small cell lung cancer and successfully detected epidermal growth factor receptor gene mutations (L858R, T790M) during targeted drug therapy. The proposed lab-on-a-disc enables a fully automated, rapid, and point-of-care cfDNA enrichment starting from whole blood to facilitate the wide use of liquid biopsy in routine clinical practice. | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.18, no.9, pp.1320 - 1329 | - |
dc.identifier.doi | 10.1039/c8lc00165k | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.scopusid | 2-s2.0-85046342246 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/24223 | - |
dc.identifier.url | http://pubs.rsc.org/en/Content/ArticleLanding/2018/LC/C8LC00165K#!divAbstract | - |
dc.identifier.wosid | 000431183100005 | - |
dc.language | 영어 | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Fully automated, on-site isolation of cfDNA from whole blood for cancer therapy monitoring | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Chemistry; Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | LIQUID BIOPSIES | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | GEFITINIB | - |
dc.subject.keywordPlus | CIRCULATING TUMOR DNA | - |
dc.subject.keywordPlus | CELL LUNG-CANCER | - |
dc.subject.keywordPlus | EGFR T790M MUTATION | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
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