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Jang, Jaesung
Sensors & Aerosols Lab.
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Cost-Effective and Handmade Paper-Based Immunosensing Device for Electrochemical Detection of Influenza Virus

Author(s)
Devarakonda, SivaranjaniSingh, RenuBhardwaj, JyotiJang, Jaesung
Issued Date
2017-11
DOI
10.3390/s17112597
URI
https://scholarworks.unist.ac.kr/handle/201301/22929
Fulltext
http://www.mdpi.com/1424-8220/17/11/2597
Citation
SENSORS, v.17, no.11, pp.2597
Abstract
Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanoparticles, and using stencil-printed electrodes. We used a glass vaporizer to spray the hydrophobic silica nanoparticles onto the paper, rendering it super-hydrophobic. The super-hydrophobicity, which is essential for this paper-based biosensor, was achieved via 30-40 spray coatings, corresponding to a 0.39-0.41 mg cm(-2) coating of nanoparticles on the paper and yielding a water contact angle of 150 degrees +/- 1 degrees. Stencil-printed carbon electrodes modified with single-walled carbon nanotubes and chitosan were employed to increase the sensitivity of the sensor, and the antibodies were immobilized via glutaraldehyde cross-linking. Differential pulse voltammetry was used to assess the sensitivity of the sensors at various virus concentrations, ranging from 10 to 10(4) PFU mL(-1), and the selectivity was assessed against MS2 bacteriophages and the influenza B viruses. These immunosensors showed good linear behaviors, improved detection times (30 min), and selectivity for the H1N1 virus with a limit of detection of 113 PFU mL(-1), which is sufficiently sensitive for rapid on-site diagnosis. The simple and inexpensive methodologies developed in this study have great potential to be used for the development of a low-cost and disposable immunosensor for detection of pathogenic microorganisms, especially in developing countries.
Publisher
MDPI AG
ISSN
1424-8220
Keyword (Author)
silica nanoparticlesinfluenza viruspaper sensorstencil printingelectrochemical immunosensorlabel-free detectioncarbon nanotubes
Keyword
LABEL-FREE DETECTIONCARBON NANOTUBESRAPID DETECTIONPLATFORMIMMUNODEVICEIMMUNOASSAYELECTRODESBIOSENSORH1N1

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