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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 117 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 114 | - |
dc.citation.title | BIODESIGN | - |
dc.citation.volume | 5 | - |
dc.contributor.author | Park, Jumi | - |
dc.contributor.author | Song, Kyonghwa | - |
dc.contributor.author | Oh, Sohyeon | - |
dc.contributor.author | Son, Taewon | - |
dc.contributor.author | Lee, Jun | - |
dc.contributor.author | Park, Ayoung | - |
dc.contributor.author | Kim, Hyun Ji | - |
dc.contributor.author | Jun, Youngsoo | - |
dc.contributor.author | Lee, Changwook | - |
dc.date.accessioned | 2023-12-21T21:44:41Z | - |
dc.date.available | 2023-12-21T21:44:41Z | - |
dc.date.created | 2017-10-26 | - |
dc.date.issued | 2017-09 | - |
dc.description.abstract | Vac8p is a vacuolar protein that plays pivotal roles in both vacuole inheritance and the formation of nucleus vacuole junction (NVJ) in yeast. The Vac8p directly interacts with Atg13p, a component of the autophagy machinery, and mediates cytoplasm-to-vacuole targeting (Cvt) pathway, resulting in the maturation of aminopeptidase I (Ape1p). Here, we coexpressed and purified Saccharomyces cerevisiae Vac8p complexed with Atg13p in Escherichia coli bacteria cells, and crystallized the complex proteins under the condition of 25% (v/v) PEG 400, 100 mM Tris pH 8.5, 2% (v/v) Ethylene glycol, 2% (w/v) PEG 3350, 1.5% (w/v) PEG 20000, 5 mM DTT at 293K. X-ray diffraction data of the crystals were collected to 2.9 Å resolution at the synchrotron radiation. The crystals belong to the orthorhombic space group P212121 with unit cell parameters a = 62.7 Å, b = 92.4 Å, and c = 139.9 Å. The asymmetric unit contains one Vac8p-Atg13p heterodimer with a corresponding VM of 2.92 Å3 Da-1 and solvent content of 57.8%. | - |
dc.identifier.bibliographicCitation | BIODESIGN, v.5, no.3, pp.114 - 117 | - |
dc.identifier.issn | 2288-6982 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/22877 | - |
dc.identifier.url | http://www.bdjn.org/Journal_Prv_View.html?j_sub_num=82 | - |
dc.language | 한국어 | - |
dc.publisher | 한국구조생물학회 | - |
dc.title | Purification, crystallization, and X-ray crystallographic analysis of Vac8p complexed with Atg13p from Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.identifier.kciid | ART002274208 | - |
dc.description.journalRegisteredClass | kci | - |
dc.description.journalRegisteredClass | kci_candi | - |
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