There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1550 | - |
dc.citation.startPage | 1542 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 245 | - |
dc.contributor.author | Lama, Suman | - |
dc.contributor.author | Seol, Eunhee | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T21:37:06Z | - |
dc.date.available | 2023-12-21T21:37:06Z | - |
dc.date.created | 2017-06-20 | - |
dc.date.issued | 2017-12 | - |
dc.description.abstract | The production of 1,3-propanediol (1,3-PDO) from glucose was investigated using Klebsiella pneumoniae J2B, which converts glycerol to 1,3-PDO and synthesize an essential coenzyme B12. In order to connect the glycolytic pathway with the pathway of 1,3-PDO synthesis from glycerol, i.e., to directly produce diol from glucose, glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate phosphatase from Saccharomyces cerevisiae were overexpressed. Additionally, the effect of expression levels and the use of isoforms of these two enzymes on glycerol and 1,3-PDO production were studied. Furthermore, to prevent loss of produced glycerol, the glycerol oxidation pathways were disrupted. Finally, the conversion rate of glycerol to 1,3-PDO was increased via homologous overexpression of glycerol dehydratase and 1,3-PDO oxidoreductase. The resultant strain successfully produced 1,3-PDO from glucose at a yield of 0.27mol/mol along with glycerol at 0.52mol/mol. Improvement of the engineered K. pneumoniae J2B to further increase conversion of glycerol to 1,3-PDO is discussed. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.245, pp.1542 - 1550 | - |
dc.identifier.doi | 10.1016/j.biortech.2017.05.052 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-85019563534 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/22864 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S096085241730706X | - |
dc.identifier.wosid | 000413038300031 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Metabolic engineering of Klebsiella pneumoniae J2B for the production of 1,3-propanediol from glucose | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.relation.journalResearchArea | Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 1,3-PDO | - |
dc.subject.keywordAuthor | Glucose | - |
dc.subject.keywordAuthor | Glycerol | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae J2B | - |
dc.subject.keywordPlus | 3-HYDROXYPROPIONIC ACID PRODUCTION | - |
dc.subject.keywordPlus | GLYCEROL-3-PHOSPHATE DEHYDROGENASE | - |
dc.subject.keywordPlus | GLYCEROL METABOLISM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | PRECISE | - |
dc.subject.keywordPlus | SYSTEM | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.