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DC Field | Value | Language |
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dc.citation.title | BMC IMMUNOLOGY | - |
dc.citation.volume | 16 | - |
dc.contributor.author | Kim, Jeong-In | - |
dc.contributor.author | Park, Tae-Eun | - |
dc.contributor.author | Maharjan, Sushila | - |
dc.contributor.author | Li, Hui-Shan | - |
dc.contributor.author | Lee, Ho-Bin | - |
dc.contributor.author | Kim, In-Seon | - |
dc.contributor.author | Piao, Dachuan | - |
dc.contributor.author | Lee, Jun-Yeong | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.contributor.author | Bok, Jin-Duck | - |
dc.contributor.author | Hong, Zhong-Shan | - |
dc.contributor.author | Kang, Sang-Kee | - |
dc.contributor.author | Choi, Yun-Jaie | - |
dc.date.accessioned | 2023-12-22T00:36:47Z | - |
dc.date.available | 2023-12-22T00:36:47Z | - |
dc.date.created | 2017-09-01 | - |
dc.date.issued | 2015-11 | - |
dc.description.abstract | Background: To initiate mucosal immune responses, antigens in the intestinal lumen must be transported into gut-associated lymphoid tissue through M cells. Recently, it has been increasingly recognized that receptor activator of NF-kB ligand (RANKL) controls M cell differentiation by interacting with RANK expressed on the sub-epithelium of Peyer's patches. In this study, we increased the number of M cells using soluble RANKL (sRANKL) as a potent mucosal adjuvant. Results: For efficient oral delivery of sRANKL, we constructed recombinant Lactococcus lactis (L. lactis) IL1403 secreting sRANKL (sRANKL-LAB). The biological activity of recombinant sRANKL was confirmed by observing RANK-RANKL signaling in vitro. M cell development in response to oral administration of recombinant L. lactis was determined by 1.51-fold higher immunohistochemical expression of M cell marker GP-2, compared to that of non-treatment group. In addition, an adjuvant effect of sRANKL was examined by immunization of mice with M-BmpB as a model antigen after treatment with sRANKL-LAB. Compared with the wild-type L. lactis group, the sRANKL-LAB group showed significantly increased systemic and mucosal immune responses specific to M-BmpB. Conclusions: Our results show that the M cell development by sRANKL-LAB can increase the antigen transcytotic capability of follicle-associated epithelium, and thereby enhance the mucosal immune response, which implies that oral administration of sRANKL is a promising adjuvant strategy for efficient oral vaccination. | - |
dc.identifier.bibliographicCitation | BMC IMMUNOLOGY, v.16 | - |
dc.identifier.doi | 10.1186/s12865-015-0132-x | - |
dc.identifier.issn | 1471-2172 | - |
dc.identifier.scopusid | 2-s2.0-84947902145 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/22642 | - |
dc.identifier.url | https://bmcimmunol.biomedcentral.com/articles/10.1186/s12865-015-0132-x | - |
dc.identifier.wosid | 000365781700002 | - |
dc.language | 영어 | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Soluble RANKL expression in Lactococcus lactis and investigation of its potential as an oral vaccine adjuvant | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Oral adjuvant | - |
dc.subject.keywordAuthor | RANKL | - |
dc.subject.keywordAuthor | M cells | - |
dc.subject.keywordAuthor | L. lactis | - |
dc.subject.keywordAuthor | Mucosal immunization | - |
dc.subject.keywordPlus | M-CELLS | - |
dc.subject.keywordPlus | MUCOSAL ADJUVANT | - |
dc.subject.keywordPlus | INFLUENZA-VIRUS | - |
dc.subject.keywordPlus | PEYERS-PATCHES | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | IMMUNITY | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | IMMUNIZATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
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